Human Inflammation 抗体 Array - Membrane (20 targets) (ab169804)
製品の概要
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製品名
Human Inflammation Antibody Array - Membrane (20 targets)
Inflammation Antibody Array antibody arrays 製品一覧 -
サンプルの種類
Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cell culture extracts, Other biological fluids, Whole Blood, Tissue Extracts, Cell Lysate, Cell culture media -
アッセイタイプ
Semi-quantitative -
種交差性
交差種: Human -
製品の概要
ab169804 detects 20 Human Inflammatory Factors in 4 or 8 samples. Suitable for all liquid sample types.
Targets: Eotaxin-1, Eotaxin-2, G-CSF, GM-CSF, IFN-gamma, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-6, IL-7, IL-8, IL-10, IL-11, IL-12 p40, IL-12 p70, IL-13, I-309, TIMP-2
Learn more about membrane antibody arrays
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特記事項
If you are interested in this cytokine array, arrays ab133996, ab133997, ab169817, ab133998 and ab134003 may also be of interest. A list of human antibody membrane arrays is available here.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
アプリケーション
適用あり: Multiplex Protein Detectionmore details
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 4 Membranes 1 x 8 Membranes 1,000X HRP-Conjugated Streptavidin 1 x 50µl 1 x 50µl 1X Blocking Buffer 1 x 25ml 2 x 25ml 2000X Biotin-Conjugated Anti Cytokines 2 vials 4 vials 20X Wash Buffer I 1 x 10ml 1 x 20ml 20X Wash Buffer II 1 x 10ml 1 x 20ml 2X Cell Lysis Buffer 1 x 10ml 1 x 16ml 8-Well Incubation Tray (with Lid) 1 unit 1 unit Detection Buffer C 1 x 1.5ml 1 x 2.5ml Detection Buffer D 1 x 1.5ml 1 x 2.5ml Inflammation Antibody Array Membranes 4 units 8 units -
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab169804の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Multiplex Protein Detection |
Use at an assay dependent concentration.
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| 特記事項 |
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Multiplex Protein Detection
Use at an assay dependent concentration. |
画像
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Multiplex Protein Detection - Human Inflammation Antibody Array - Membrane (20 targets) (ab169804)
Human peripheral blood cells (1x106 cells/mL) were cultured in RPMI media supplemented with 10% fetal calf serum, 100 U/mL penicillin, and 100 mg/mL streptomycin sulfate.
Cells were cultured unstimulated or stimulated with 10 mg/mL PHA. Conditioned media was harvested after 48 hours, aliquoted and assayed using ab169804. Media alone was used as a negative control.
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Quantification of Human Inflammation Antibody ArrayCells were cultured unstimulated or stimulated with 10 mg/mL PHA. Conditioned media was harvested after 48 hours, aliquoted and assayed using ab169804. Media alone was used as a negative control. Mean pixel density was quantified using CCD camera software analysis.
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Multiplex Protein Detection - Human Inflammation Antibody Array - Membrane (20 targets) (ab169804)HepG2 cells (Human hepatocellular carcinoma) were seeded at 1x106 cells/mL and cultured in EMEM media supplemented with 10% fetal calf serum, 100 U/mL penicillin, and 100 µg/mL streptomycin sulfate. Conditioned media was harvested after 48 hours, aliquoted and assayed using ab169804.
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Quantification of Human Inflammation Antibody ArrayConditioned media was harvested after 48 hours, aliquoted and assayed using ab169804. Mean pixel density was quantified using CCD camera software analysis.
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Multiplex Protein Detection - Human Inflammation Antibody Array - Membrane (20 targets) (ab169804)Typical results obtained with Abcam Membrane Antibody Arrays. Image above presents typical images obtained with Abcam Human Cytokine Membrane Antibody Array. These membranes were probed with conditioned media from two different cell lines. Membranes were exposed to film at RT for 1 min.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab169804 は論文での使用が確認できていません。