Mouse specific HRP/DAB Detection IHC Kit (ab64259)
製品の概要
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製品名
Mouse specific HRP/DAB Detection IHC Kit
HRP/DAB detection kit キット 製品一覧 -
製品の概要
Mouse specific HRP/DAB IHC Detection Kit ab64259 is a complete kit to detect a primary antibody raised in mouse, using HRP-labeled-streptavidin and a biotinylated anti-mouse secondary antibody.
It is supplied with a full protocol and with peroxidase and protein blocking reagents, biotinylated secondary antibody, streptavidin-HRP, and DAB substrate.
In the method:
- a sample is incubated with an unconjugated primary antibody specific to the target antigen
- a biotinylated secondary antibody then binds to the primary antibody
- HRP-labeled streptavidin then binds to the secondary antibody
- the HRP produces a brown colored substance at the site of primary antibody binding by reacting with DAB. -
特記事項
For alternative streptavidin-biotin IHC detection kits see:
- Anti-Mouse / anti-Rabbit HRP-DAB IHC kit ab64264
- Anti-Rabbit HRP-DAB IHC Kit ab64261For high sensitivity HRP micro-polymer IHC detection kits see:
- Anti-Mouse / anti-Rabbit HRP/DAB Micro-polymer IHC Kit ab236466
- Anti-Rabbit HRP-DAB Micro-polymer IHC Kit ab236469
Alternatively, see HRP-AEC IHC detection kits.For staining mouse tissues with mouse antibodies, use mouse-on-mouse polymer kit ab127055.
Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.
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アプリケーション
適用あり: IHC-Pmore details
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 15 ml 50x DAB Chromogen 1 x 0.5ml Biotinylated goat anti-mouse IgG(H+L) (ab64255) 1 x 15ml DAB substrate 1 x 15ml Hydrogen Peroxide Block 1 x 15ml Protein block 1 x 15ml Streptavidin Peroxidase 1 x 15ml -
研究分野
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関連性
HRP/DAB detection kit is a immunoenzymatic antigen detection system for immunohistochemistry techniques.
関連製品
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Related Buffer
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab64259の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
Use at an assay dependent dilution.
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| 特記事項 |
|---|
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IHC-P
Use at an assay dependent dilution. |
画像
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Immunohistochemistry - ab64259Jovicic N et al., PLoS One 10(7). Fig 6D & E. DOI: 10.1371/journal.pone.0134089. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
Immunohistochemical analysis staining SMA or CD68 in paraffin embedded mouse liver tissue sections Samples were incubated with anti-α- SMA (ab7817) or anti-CD68 (ab49777). A Mouse specific HRP/DAB Detection IHC Kit (AB64259 was used as a stain and Mayer’s hematoxylin was used as a counterstain. Top panel of images staining CD68 and bottom panel staining α-SMA.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti Mouse HRP/DAB Detection Kit (ab64259)Human colon carcinoma stained with anti p53 antibody using Anti Mouse HRP/DAB Detection Kit (ab64259).
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (49)
ab64259 は 49 報の論文で使用されています。
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- Rusu B et al. Single-Nucleus Profiling Identifies Accelerated Oligodendrocyte Precursor Cell Senescence in a Mouse Model of Down Syndrome. eNeuro 10:N/A (2023). PubMed: 37491366