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Mouse Interferon gamma ELISPOT Kit (with un-coated plates) (ab46587)

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Key features and details

  • Assay type: Sandwich (qualitative)
  • Detection method: Colorimetric
  • Sample type: Suspension cells
  • Reacts with: Mouse

製品の概要

  • 製品名

    Mouse Interferon gamma ELISPOT Kit (with un-coated plates)
    Interferon gamma キット 製品一覧

  • 検出方法

    Colorimetric

  • サンプルの種類

    Suspension cells

  • アッセイタイプ

    Sandwich (qualitative)

  • ステップ

    Multiple steps standard assay

  • 種交差性

    交差種: Mouse

  • 製品の概要

    The Mouse Interferon gamma ELISPOT Kit (with un-coated plates) is designed to enumerate cytokine producing cells in a single cell suspension. This method has the advantage of requiring a minimum of in-vitro manipulations allowing cytokine production analysis as close as possible to in-vivo conditions in a highly specific way. This technique is designed to determine the frequency of cytokine producing cells under a given stimulation, and the follow-up of such frequency during a treatment and/or a pathological state. Elispot assay constitutes an ideal tool in the TH1 / TH2 response, vaccine development, viral infection monitoring and treatment, cancerology, infectious diseases, autoimmune diseases and transplantation.


    This ELISPOT assay is based on sandwich immuno-enzyme technology. Cell secreted cytokines or soluble molecules are captured by coated antibodies avoiding diffusion in supernatant, protease degradation or binding on soluble membrane receptors. After cell removal, the captured cytokines are revealed by tracer antibodies and appropriate conjugates.


    Principal:


    After cell stimulation, locally produced cytokines are captured by a specific monoclonal antibody. After cell lysis, trapped cytokine molecules are revealed by a secondary biotinylated detection antibody, which is in turn recognised by streptavidin conjugated to alkaline phosphatase. PVDF-bottomed-well plates are then incubated with BCIP/NBT substrate. Colored "purple" spots indicate cytokine production by individual cells.


    Recognizes natural murine Interferon Gamma.

  • アプリケーション

    適用あり: ELISpotmore details

  • 試験プラットフォーム

    Microplate

製品の特性

  • 保存方法

    Store at +4°C. Please refer to protocols.
  • 内容 5 x 96 tests 10 x 96 tests
    96 PVDF-bottomed-well plates. 5 units 10 units
    Bovine Serum Albumin 1 x 1g 2 x 1g
    IFNγ Biotinylated detection antibody 1 vial 2 vials
    Clone DB1 Capture antibody 1 x 500µl 2 x 500µl
    Ready-to-use BCIP/NBT substrate buffer 1 x 50ml 2 x 50ml
    Streptavidin - Alkaline Phosphatase conjugated 1 x 50µl 2 x 50µl

  • 研究分野

  • 機能

    Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.

  • 組織特異性

    Released primarily from activated T lymphocytes.

  • 関連疾患

    In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.

  • 配列類似性

    Belongs to the type II (or gamma) interferon family.

  • 翻訳後修飾

    Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.

  • 細胞内局在

    Secreted.
  • Information by UniProt

  • 別名

    • IF 1
    • IFG
    • IFI
    • ifn g
    • IFN gamma
    • IFN immune
    • IFN, immune
    • IFN-gamma
    • IFNG
    • IFNG_HUMAN
    • Immune interferon
    • Interferon gamma
    • Type II Interferon
    see all

  • 参照データベース

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab46587の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    ELISpot
    Use at an assay dependent dilution.
    特記事項
    ELISpot
    Use at an assay dependent dilution.

    参考文献 (1)

    ab46587 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab46587 は 1 報の論文で使用されています。

    • Xu M  et al. Hollow mesoporous ruthenium nanoparticles conjugated bispecific antibody for targeted anti-colorectal cancer response of combination therapy. Nanoscale 11:9661-9678 (2019). PubMed: 31065660

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