Human Interferon Gamma ELISPOT Kit (ab62899)
Key features and details
- Assay type: Sandwich (qualitative)
- Detection method: Colorimetric
- Sample type: Suspension cells
- Reacts with: Human
製品の概要
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製品名
Human Interferon Gamma ELISPOT Kit
Interferon gamma キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Suspension cells -
アッセイタイプ
Sandwich (qualitative) -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
The ELISPOT assay is designed to enumerate cytokine producing cells in a single cell suspension. This method has the advantage of requiring a minimum of in-vitro manipulations allowing cytokine production analysis as close as possible to in-vivo conditions in a highly specific way. This technique is designed to determine the frequency of cytokine producing cells under a given stimulation, and the follow-up of such frequency during a treatment and/or a pathological state. Elispot assay constitutes an ideal tool in the TH1 / TH2 response, vaccine development, viral infection monitoring and treatment, cancerology, infectious diseases, autoimmune diseases and transplantation.
Abcam Elispot assay is based on sandwich immuno-enzyme technology. Cell secreted cytokines or soluble molecules are captured by coated antibodies avoiding diffusion in supernatant, protease degradation or binding on soluble membrane receptors. After cell removal, the captured cytokines are revealed by tracer antibodies and appropriate conjugates.
Principal:
After cell stimulation, locally produced cytokines are captured by a specific monoclonal antibody. After cell lysis, trapped cytokine molecules are revealed by a secondary biotinylated detection antibody, which is in turn recognised by streptavidin conjugated to alkaline phosphatase. PVDF-bottomed-well plates are then incubated with BCIP/NBT substrate. Colored "purple" spots indicate cytokine production by individual cells.
Recognizes natural human Interferon gamma
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アプリケーション
適用あり: ELISpotmore details -
試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 5 x 96 tests Biotinylated Human IFNγ Detection Antibody 1 vial 1 vial Bovine Serum Albumin 1 x 2g 1 x 2g IFNγ Pre-coated 96-well PDVF-bottomed plates 1 unit 5 units Ready-to-use BCIP/NBT substrate buffer 1 x 11ml 2 x 25ml Streptavidin - Alkaline Phosphatase conjugated 1 x 10µl 1 x 50µl -
研究分野
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機能
Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. -
組織特異性
Released primarily from activated T lymphocytes. -
関連疾患
In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis. -
配列類似性
Belongs to the type II (or gamma) interferon family. -
翻訳後修飾
Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161. -
細胞内局在
Secreted. - Information by UniProt
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別名
- IF 1
- IFG
- IFI
see all -
参照データベース
- Entrez Gene: 3458 Human
- Omim: 147570 Human
- SwissProt: P01579 Human
- Unigene: 856 Human
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab62899の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ELISpot |
Use at an assay dependent dilution.
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| 特記事項 |
|---|
|
ELISpot
Use at an assay dependent dilution. |
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab62899 は論文での使用が確認できていません。