Human Interferon Gamma + IL2 FLUOROSPOT Kit (ab48452)
Key features and details
- Assay type: Sandwich (qualitative)
- Detection method: Fluorescent
- Sample type: Suspension cells
- Reacts with: Human
製品の概要
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製品名
Human Interferon Gamma + IL2 FLUOROSPOT Kit
Interferon Gamma + IL2 キット 製品一覧 -
検出方法
Fluorescent -
サンプルの種類
Suspension cells -
アッセイタイプ
Sandwich (qualitative) -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
The ELISPOT assay is designed to enumerate cytokine producing cells in a single cell suspension. This method has the advantage of requiring a minimum of in-vitro manipulations allowing cytokine production analysis as close as possible to in-vivo conditions in a highly specific way. This technique is designed to determine the frequency of cytokine producing cells under a given stimulation, and the follow-up of such frequency during a treatment and/or a pathological state. Elispot assay constitutes an ideal tool in the TH1 / TH2 response, vaccine development, viral infection monitoring and treatment, oncology, infectious diseases, autoimmune diseases and transplantation.
This Elispot assay is based on sandwich immuno-enzyme technology. Cell secreted cytokines or soluble molecules are captured by coated antibodies avoiding diffusion in supernatant, protease degradation or binding on soluble membrane receptors. After cell removal, the captured cytokines are revealed by tracer antibodies and appropriate conjugates.
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アプリケーション
適用あり: FluroSpotmore details -
試験プラットフォーム
Microplate
製品の特性
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保存方法
Please refer to protocols. -
内容 1 x 96 tests 5 x 96 tests 10 x 96 tests 96 well PVDF bottomed Microplate (non-sterile) 1 unit 5 units 10 units Anti-FITC antibody green fluorescence conjugate. 1 vial 1 vial 2 vials Biotinylated detection antibody for IL-2 (resuspend in 0.55mL). 1 vial 1 vial 2 vials Bovine Serum Albumin 1 x 2g 1 x 2g 2 x 2g FITC conjugated detection antibody for IFNg (resuspend in 0.55mL) 1 vial 1 vial 2 vials Human IFN Gamma Capture antibody 1 x 100µl 1 x 500µl 2 x 500µl Human IL-2 Capture antibody 1 x 100µl 1 x 500µl 2 x 500µl Streptavidin-phycoerythrin conjugate. 1 vial 1 vial 2 vials -
研究分野
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関連性
IFN-gamma is produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. -
細胞内局在
Secreted -
別名
- IFN gamma
- IFNG
- IL2
see all -
参照データベース
- Entrez Gene: 3458 Human
- Entrez Gene: 3558 Human
- SwissProt: P01579 Human
- SwissProt: P60568 Human
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab48452の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| FluroSpot |
Use at an assay dependent dilution.
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| 特記事項 |
|---|
|
FluroSpot
Use at an assay dependent dilution. |
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab48452 は 1 報の論文で使用されています。
- Kuloğlu ZE et al. Effect of BTN162b2 and CoronaVac boosters on humoral and cellular immunity of individuals previously fully vaccinated with CoronaVac against SARS-CoV-2: A longitudinal study. Allergy 77:2459-2467 (2022). PubMed: 35437772