Human Interferon Gamma + IL2 ELISPOT Kit (ab48448)
Key features and details
- Assay type: Sandwich (qualitative)
- Detection method: Colorimetric
- Sample type: Suspension cells
- Reacts with: Human
製品の概要
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製品名
Human Interferon Gamma + IL2 ELISPOT Kit
Interferon Gamma + IL2 キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Suspension cells -
アッセイタイプ
Sandwich (qualitative) -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
The ELISPOT assay is designed to enumerate cytokine producing cells in a single cell suspension. This method has the advantage of requiring a minimum of in-vitro manipulations allowing cytokine production analysis as close as possible to in-vivo conditions in a highly specific way. This technique is designed to determine the frequency of cytokine producing cells under a given stimulation, and the follow-up of such frequency during a treatment and/or a pathological state. Elispot assay constitutes an ideal tool in the TH1 / TH2 response, vaccine development, viral infection monitoring and treatment, oncology, infectious diseases, autoimmune diseases and transplantation.
This Elispot assay is based on sandwich immuno-enzyme technology. Cell secreted cytokines or soluble molecules are captured by coated antibodies avoiding diffusion in supernatant, protease degradation or binding on soluble membrane receptors. After cell removal, the captured cytokines are revealed by tracer antibodies and appropriate conjugates.
The dual colour Elispot allows you to monitor the production of two cytokines simultaneously in the same well.
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アプリケーション
適用あり: ELISpotmore details -
試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 5 x 96 tests 10 x 96 tests 10 x concentrate buffer for the preparation of AEC buffer 1 x 5ml 2 x 5ml 50 x concentrate AEC substrate buffer 1 x 1ml 2 x 1ml 96 PVDF-bottomed-well plates. 5 units 10 units Anti-FITC antibody HRP conjugate 1 x 120µl 2 x 100µl Bovine Serum Albumin 1 x 1g 2 x 1g Human IFN Gamma Capture antibody 1 x 500µl 2 x 500µl Human IL-2 Capture antibody 1 x 500µl 2 x 500µl IFNγ FITC conjugated detection antibody 1 vial 2 vials IL-2 Biotinylated detection antibody 1 vial 2 vials Ready-to-use BCIP/NBT substrate buffer 2 x 27ml 2 x 50ml Streptavidin - Alkaline Phosphatase conjugated 1 x 50µl 2 x 50µl -
研究分野
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関連性
IFN-gamma is produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. -
細胞内局在
Secreted -
別名
- IFN gamma
- IFNG
- IL2
see all -
参照データベース
- Entrez Gene: 3458 Human
- Entrez Gene: 3558 Human
- SwissProt: P01579 Human
- SwissProt: P60568 Human
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab48448の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ELISpot |
Use at an assay dependent dilution.
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| 特記事項 |
|---|
|
ELISpot
Use at an assay dependent dilution. |
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab48448 は論文での使用が確認できていません。