Human IL-6 ELISPOT Kit (ab62936)
Key features and details
- Assay type: Sandwich (qualitative)
- Detection method: Colorimetric
- Sample type: Suspension cells
- Reacts with: Human
製品の概要
-
製品名
Human IL-6 ELISPOT Kit
IL-6 キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Overall 12 5.9% Overall 12 3.9% Overall 12 4.7% Overall 12 6.3% Overall 12 10.4% -
サンプルの種類
Suspension cells -
アッセイタイプ
Sandwich (qualitative) -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Human IL-6 ELISpot (ab62936) is a highly specific immunoassay for the analysis of IL-6 production and secretion from T-cells at a single cell level in conditions closely comparable to the in-vivo environment with minimal cell manipulation.
A capture antibody highly specific for the analyte of interest is coated to the wells of a PVDF bottomed 96 well microtiter plate either during kit manufacture or in the laboratory. The plate is then blocked to minimise any non-antibody dependent unspecific binding and washed. Cell suspension and stimulant are added and the plate incubated allowing the specific antibodies to bind any analytes produced. Cells are then removed by washing prior to the addition of Biotinylated detection antibodies which bind to the previously captured analyte. Enzyme conjugated streptavidin is then added binding to the detection antibodies. Following incubation and washing substrate is then applied to the wells resulting in coloured spots which can be quantified using appropriate analysis software or manually using a microscope.
For the quantitative determination of the frequency of Human IL-6 -producing cells.
For research use only - not intended for diagnostic use.
-
アプリケーション
適用あり: ELISpotmore details -
試験プラットフォーム
Pre-coated microplate (12 x 8 well strips)
製品の特性
-
保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests Biotinylated detection antibody, clone B-E4: 1 x 100µl Bovine Serum Albumin 1 x 2g Human IL-6 Pre-coated 96-well PDVF-bottomed plates 1 unit Ready-to-use BCIP/NBT substrate buffer 1 x 11ml Streptavidin - Alkaline Phosphatase conjugated 1 x 10µl -
研究分野
-
機能
Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. -
関連疾患
Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men. -
配列類似性
Belongs to the IL-6 superfamily. -
翻訳後修飾
N- and O-glycosylated. -
細胞内局在
Secreted. - Information by UniProt
-
別名
- Interleukin BSF 2
- B cell differentiation factor
- B cell stimulatory factor 2
see all -
参照データベース
- Entrez Gene: 3569 Human
- Omim: 147620 Human
- SwissProt: P05231 Human
- Unigene: 654458 Human
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab62936の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ELISpot |
Use at an assay dependent dilution.
|
| 特記事項 |
|---|
|
ELISpot
Use at an assay dependent dilution. |
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab62936 は論文での使用が確認できていません。