Human IL-17 ELISPOT Set (ab83712)
Key features and details
- Assay type: Sandwich (qualitative)
- Sample type: Suspension cells
- Reacts with: Human
製品の概要
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製品名
Human IL-17 ELISPOT Set
IL-17 キット 製品一覧 -
サンプルの種類
Suspension cells -
アッセイタイプ
Sandwich (qualitative) -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Intended Use
The ELISPOT assay is designed to enumerate cytokine producing cells in a single cell suspension. This method has the advantage of requiring a minimum of in-vitro manipulations allowing cytokine production analysis as close as possible to in-vivo conditions in a highly specific way. This technique is designed to determine the frequency of cytokine producing cells under a given stimulation, and the follow-up of such frequency during a treatment and/or a pathological state. ELISPOT assay constitutes an ideal tool in the TH1 / TH2 response, vaccine development, viral infection monitoring and treatment, cancerology, infectious diseases, autoimmune diseases and transplantation. The ELISPOT assay is based on sandwich immuno-enzyme technology. Cell secreted cytokines or soluble molecules are captured by coated antibodies avoiding diffusion in supernatant, protease degradation or binding on soluble membrane receptors. After cell removal, the captured cytokines are revealed by tracer antibodies and appropriate conjugates.
Principle of Method
After cell stimulation, locally produced cytokines are captured by a specific monoclonal antibody. After cell lysis, trapped cytokine molecules are revealed by a secondary biotinylated detection antibody, which is in turn recognised by streptavidin conjugated to alkaline phosphatase. PVDF-bottomed-well plates are then incubated with BCIP/NBT substrate. Colored "purple" spots indicate cytokine production by individual cells.
Recognizes natural human IL-17A.
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アプリケーション
適用あり: ELISpotmore details -
試験プラットフォーム
Reagents
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 5 x 96 tests 10 x 96 tests Bovine Serum Albumin 1 x 1g 2 x 1g Human IL-17 Biotinylated detection antibody 1 vial 1 vial Human IL-17 Capture antibody 1 x 500µl 2 x 500µl Ready-to-use BCIP/NBT substrate buffer 1 x 50ml 2 x 50ml Streptavidin - Alkaline Phosphatase conjugated 1 x 50µl 2 x 50µl -
研究分野
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関連性
IL-17, also known as CTLA8, is a T cell derived hematopoietic cytokine. It was originally cloned from a T cell hybridoma produced by fusion of a mouse cytotoxic T cell clone and a rat T lymphoma. IL-17 exhibits multiple biological activities on a variety of cells including: the induction of IL-6, IL-8 and GCSF production in fibroblasts; the enhancement of surface expression of ICAM 1 in fibroblasts; activation of NF kappaB and costimulation of T cell proliferation. IL-17 is an approximately 16 kDa polypeptide of 136 amino acids. The precursor form of IL-17 consists of 155 amino acids. To generate the mature IL-17 (136 amino acids), the precursor cleaves a 19 amino acid signal peptide. Human IL-17 shows approximately 62.5% amino acid homology to mouse IL-17 and 58% amino acid homology to rat IL-17. -
細胞内局在
Secreted -
別名
- CTLA 8
- CTLA8
- Cytotoxic T lymphocyte associated antigen 8
see all -
参照データベース
- Entrez Gene: 3605 Human
- Omim: 603149 Human
- SwissProt: Q16552 Human
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab83712の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ELISpot |
Use at an assay dependent dilution.
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| 特記事項 |
|---|
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ELISpot
Use at an assay dependent dilution. |
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab83712 は 1 報の論文で使用されています。
- Dissanayake K et al. Potential applicability of cytokines as biomarkers of disease activity in rheumatoid arthritis: Enzyme-linked immunosorbent spot assay-based evaluation of TNF-a, IL-1ß, IL-10 and IL-17A. PLoS One 16:e0246111 (2021). PubMed: 33497394