Human IL-13 ELISPOT Set (ab46580)
Key features and details
- Assay type: Sandwich (qualitative)
- Sample type: Suspension cells
- Reacts with: Human
製品の概要
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製品名
Human IL-13 ELISPOT Set
IL-13 キット 製品一覧 -
サンプルの種類
Suspension cells -
アッセイタイプ
Sandwich (qualitative) -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
The Human IL-13 ELISPOT Set is designed to enumerate cytokine producing cells in a single cell suspension. This method has the advantage of requiring a minimum of in-vitro manipulations allowing cytokine production analysis as close as possible to in-vivo conditions in a highly specific way. This technique is designed to determine the frequency of cytokine producing cells under a given stimulation, and the follow-up of such frequency during a treatment and/or a pathological state. Elispot assay constitutes an ideal tool in the TH1 / TH2 response, vaccine development, viral infection monitoring and treatment, cancerology, infectious diseases, autoimmune diseases and transplantation.
This ELISPOT assay is based on sandwich immuno-enzyme technology. Cell secreted cytokines or soluble molecules are captured by coated antibodies avoiding diffusion in supernatant, protease degradation or binding on soluble membrane receptors. After cell removal, the captured cytokines are revealed by tracer antibodies and appropriate conjugates.
Principal:
After cell stimulation, locally produced cytokines are captured by a specific monoclonal antibody. After cell lysis, trapped cytokine molecules are revealed by a secondary biotinylated detection antibody, which is in turn recognised by streptavidin conjugated to alkaline phosphatase. PVDF-bottomed-well plates are then incubated with BCIP/NBT substrate. Colored "purple" spots indicate cytokine production by individual cells.
Recognizes both natural and recombinant human IL-13.
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アプリケーション
適用あり: ELISpotmore details -
試験プラットフォーム
Reagents
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 5 x 96 tests 10 x 96 tests 15 x 96 tests 20 x 96 tests Bovine Serum Albumin 1 x 1g 2 x 1g 3 x 1g 4 x 1g Dry Skimmed milk 1 vial 2 vials 3 vials 4 vials Clone B-P6 Biotinylated detection antibody, 1 vial 2 vials 3 vials 4 vials Clone B-B13 Capture antibody 1 x 500µl 2 x 500µl 3 x 500µl 4 x 500µl Ready-to-use BCIP/NBT substrate buffer 1 x 50ml 2 x 50ml 3 x 50ml 4 x 50ml Streptavidin - Alkaline Phosphatase conjugated 1 x 50µl 2 x 50µl 3 x 50µl 4 x 50µl -
研究分野
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関連性
IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells. It is involved in several stages of B-cell maturation and differentiation. It up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. IL-13 down-regulates macrophage activity, thereby inhibits the production of pro-inflammatory cytokines and chemokines. It is found to be critical to the pathogenesis of allergen-induced asthma but operates through mechanisms independent of IgE and eosinophils. IL-13, IL-3, IL-5, IL-4, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL-4. -
細胞内局在
Secreted -
別名
- ALRH
- BHR1
- IL 13
see all -
参照データベース
- Entrez Gene: 3596 Human
- Omim: 147683 Human
- SwissProt: P35225 Human
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab46580の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ELISpot |
Use at an assay dependent dilution.
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| 特記事項 |
|---|
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ELISpot
Use at an assay dependent dilution. |
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab46580 は論文での使用が確認できていません。