Anti-Thyroid Peroxidase ELISA Kit (ab178632)
Key features and details
- Sensitivity: 0.5 U/ml
- Range: 5 U/ml - 320 U/ml
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Indirect
- Reacts with: Human
製品の概要
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製品名
Anti-Thyroid Peroxidase ELISA Kit -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Pos. Serum 20 3.8% Pos. Serum 20 4.8% Neg. Serum 20 6% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Pos. Serum 12 113.3U/ml 7.1% Pos. Serum 12 176U/ml 5.7% -
サンプルの種類
Serum, Hep Plasma, Cit plasma -
アッセイタイプ
Indirect -
検出感度
< 0.5 U/ml -
検出範囲
5 U/ml - 320 U/ml -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s anti-Thyroid Peroxidase Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative determination of autoantibodies to thyroid peroxidase in Human serum or plasma (citrate).
A 96-well plate has been precoated with recombinant thyroid peroxidase to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized thyroid peroxidase-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The intensity of yellow coloration is directly proportional to the amount of thyroid peroxidase sample captured in plate.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 ラベル 1 x 96 tests 20X Washing Solution White cap 1 x 50ml Cover Foil 1 unit IgG Sample Diluent 1 x 100ml Stop Solution red cap 1 x 15ml Strip holder 1 unit Thyroid peroxidase Anti-IgG HRP Conjugate 1 x 20ml Thyroid peroxidase Coated Microplate (12 x 8 wells) 1 unit Thyroid peroxidase Negative Control 1 x 2ml Thyroid peroxidase Positive Control 1 x 2ml Thyroid peroxidase Standard A - 0 U/mL 1 x 2ml Thyroid peroxidase Standard B - 5 U/mL 1 x 2ml Thyroid peroxidase Standard C - 10 U/mL 1 x 2ml Thyroid peroxidase Standard D - 20 U/mL 1 x 2ml Thyroid peroxidase Standard E - 80 U/mL 1 x 2ml Thyroid peroxidase Standard F - 320 U/mL 1 x 2ml TMB Substrate Solution Yellow cap 1 x 15ml -
研究分野
画像
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Typical Standard Curve
Representative standard curve using ab178632
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab178632 は 2 報の論文で使用されています。
- Avalos-Díaz E et al. Multiple autoimmunity and epitope spreading in monozygotic twins. J Transl Autoimmun 4:100132 (2021). PubMed: 34816112
- Landriscina M et al. RAS/BRAF mutational status in familial non-medullary thyroid carcinomas: A retrospective study. Oncol Lett 10:1875-1881 (2015). PubMed: 26622768