STAT5 A/B pY694/699 ELISA Kit (ab176656)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 5 µg/ml
- Range: 10 µg/ml - 1000 µg/ml
- Sample type: Adherent cells, Cell culture extracts, Suspension cells, Tissue Homogenate
- Detection method: Colorimetric
- Assay type: Semi-quantitative
- Reacts with: Human
製品の概要
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製品名
STAT5 A/B pY694/699 ELISA Kit
STAT5 キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% A431 extract 6 2.8% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% A431 extract 3 4% -
サンプルの種類
Cell culture extracts, Adherent cells, Suspension cells, Tissue Homogenate -
アッセイタイプ
Semi-quantitative -
検出感度
< 5 µg/ml -
検出範囲
10 µg/ml - 1000 µg/ml -
全工程の試験時間
1h 30m -
ステップ
One step assay -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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製品の概要
Abcam’s STAT5 A/B (pY694/699) in vitro SimpleStep ELISA™ (Enzyme-Linked Immunosorbent Assay) kit is designed for the semi-quantitative measurement of STAT5 A/B (pY694/699) protein in Human cells.
The SimpleStep ELISA™ employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm
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特記事項
Abcam’s STAT5 A/B (pY694/699) SimpleStep ELISA kit (ab176656) has been re-developed with new detector antibody. From February 18, 2016 the kit will be delivered with a new detector total STAT5 antibody. This change was necessary because of a decrease in the performance of the polyclonal STAT5 antibody that was previously used for detection in recent production lots. We have identified a new antibody to use in the SimpleStep ELISA platform that provides higher performance.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 10X Wash Buffer PT 1 x 15ml 50X Cell Extraction Enhancer Solution 1 x 1ml 5X Cell Extraction Buffer PTR 1 x 12ml Lyophilized STAT5 A/B Control Lysate 1 vial Plate Seal 1 unit SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit STAT5 A/B (pY694/699) Capture Antibody 1 x 3ml STAT5 A/B (pY694/699) Detector Antibody 1 x 3ml Stop Solution 1 x 12ml TMB Substrate 1 x 12ml -
研究分野
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機能
Carries out a dual function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription. -
配列類似性
Belongs to the transcription factor STAT family.
Contains 1 SH2 domain. -
翻訳後修飾
Tyrosine phosphorylated in response to IL-2, IL-3, IL-7, IL-15, GM-CSF, growth hormone, prolactin, erythropoietin and thrombopoietin. Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity. -
細胞内局在
Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation. - Information by UniProt
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別名
- MGF
- Signal transducer and activator of transcription 5A
- Signal Transducer and Activator of Transcription 5B
see all -
参照データベース
- Entrez Gene: 6777 Human
- Entrez Gene: 6776 Human
- Entrez Gene: 20851 Mouse
- Entrez Gene: 20850 Mouse
- Entrez Gene: 25126 Rat
- Entrez Gene: 24918 Rat
- Omim: 601511 Human
- Omim: 604260 Human
see all
画像
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Other - STAT5 A/B pY694/699 ELISA Kit (ab176656)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Typical cell lysate dilution seriesExample of a typical STAT5 A/B (pY694/699) cell lysate dilution series. Background-subtracted data values (mean +/- SD) are graphed.
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Induction of STAT5 A/B (pY694/699) phosphorylation in A431 cells in response to EGF treatment.A431 cells were cultured in 96-well tissue culture plates and treated (15 min) with a dose-range of EGF before cell lysis. Data from quadruplicate measurements of STAT5 A/B (pY694/699) are plotted and compared against Total STAT5 A/B levels.
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Cell line analysis for Total STAT5 A/BCell line analysis for Total STAT5 A/B from 100 µg/mL preparations of cell extracts. Data from triplicate measurements (mean +/- SD) are plotted and compared to 1X Cell Extraction Buffer PTR (zero).
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Sandwich ELISA - STAT5 A/B pY694/699 ELISA Kit (ab176656)To learn more about the advantages of SimpleStep ELISA® kits see here.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab176656 は論文での使用が確認できていません。