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Rat sE-Selectin ELISA Kit (sCD62E) (ab171334)

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Typical standard curve

    Key features and details

    • Sensitivity: 10 pg/ml
    • Range: 156 pg/ml - 10000 pg/ml
    • Sample type: Cell culture supernatant, Cit plasma, Hep Plasma, Serum
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Rat

    製品の概要

    • 製品名

      Rat sE-Selectin ELISA Kit (sCD62E)
      CD62E キット 製品一覧

    • 検出方法

      Colorimetric

    • 再現性

      Intra-Assay（同時再現性）
      サンプル N 平均値 SD CV%
      1 16 345pg/ml 17.68 = 4.4%
      2 16 1737pg/ml 132.01 = 7.6%
      3 16 4428pg/ml 292.24 = 6.6%
      Inter-Assay（日差再現性）
      サンプル N 平均値 SD CV%
      1 24 14.8pg/ml 14.8 = 4.7%
      2 24 149.13pg/ml 149.13 = 9.2%
      3 24 309.85pg/ml 309.85 = 7.6%

    • サンプルの種類

      Cell culture supernatant, Serum, Hep Plasma, Cit plasma

    • アッセイタイプ

      Sandwich (quantitative)

    • 検出感度

      < 10 pg/ml

    • 検出範囲

      156 pg/ml - 10000 pg/ml

    • ステップ

      Multiple steps standard assay

    • 種交差性

      交差種: Rat

    • 製品の概要

      Abcam’s rat soluble sE-Selectin (sCD62E) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of rat soluble sE-Selectin in cell culture supernatants, serum and plasma (heparin, citrate).

      An sE-Selectin specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for sE-Selectin is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the rat sE-Selectin amount of sample captured in plate.

    • 試験プラットフォーム

      Microplate

    製品の特性

    画像

    • Typical standard curve
      Typical standard curve

      Representative Standard Curve using ab171334.

    参考文献 (1)

    ab171334 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab171334 は 1 報の論文で使用されています。

    • Mahmoud-Awny M  et al. Mangiferin Mitigates Gastric Ulcer in Ischemia/ Reperfused Rats: Involvement of PPAR-?, NF-?B and Nrf2/HO-1 Signaling Pathways. PLoS One 10:e0132497 (2015). PubMed: 26196679

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