Rat MIP-1 beta ELISA Kit (CCL4) (ab171336)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 15.6 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
製品の概要
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製品名
Rat MIP-1 beta ELISA Kit (CCL4)
CCL4/MIP-1 beta キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% 1 16 31pg/ml 1.42 = 4.6% 2 16 144pg/ml 9.21 = 6.4% 3 16 453pg/ml 24.31 = 5.5% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% 1 24 31pg/ml 1.67 = 5.4% 2 24 148pg/ml 12.13 = 8.2% 3 24 419pg/ml 24.3 = 5.8% -
サンプルの種類
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 10 pg/ml -
検出範囲
15.6 pg/ml - 1000 pg/ml -
ステップ
Multiple steps standard assay -
種交差性
交差種: Rat -
製品の概要
Abcam’s rat MIP-1 beta (CCL4) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of rat MIP-1 beta in cell culture supernatants, serum and plasma (heparin, EDTA, citrate)
A MIP-1 beta specific rat monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for MIP-1 beta is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the rat MIP-1 beta amount of sample captured in plate.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 ラベル 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml Antibody Diluent Buffer Green Cap 1 x 12ml Anti-rat MIP-1 beta Antibody Microplate (12 x 8 wells) 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl Biotinylated anti-rat MIP-1 beta antibody 1 x 100µl Lyophilized recombinant rat MIP-1 beta standard 2 x 10ng Plate Seal 4 units Sample Diluent Buffer Green Cap 1 x 30ml TMB Color Developing Agent Amber Bottle 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml -
研究分野
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機能
Monokine with inflammatory and chemokinetic properties. Binds to CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-beta induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form MIP-1-beta(3-69) retains the abilities to induce down-modulation of surface expression of the chemokine receptor CCR5 and to inhibit the CCR5-mediated entry of HIV-1 in T-cells. MIP-1-beta(3-69) is also a ligand for CCR1 and CCR2 isoform B. -
配列類似性
Belongs to the intercrine beta (chemokine CC) family. -
翻訳後修飾
N-terminal processed form MIP-1-beta(3-69) is produced by proteolytic cleavage after secretion from peripheral blood lymphocytes. -
細胞内局在
Secreted. - Information by UniProt
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別名
- MIP 1 beta
- Secreted protein G 26
- ACT 2
see all -
参照データベース
- Entrez Gene: 116637 Rat
- SwissProt: P50230 Rat
画像
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Typical standard curve
Representative Standard Curve using ab171336.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab171336 は 1 報の論文で使用されています。
- Bi D et al. Effects of leukotriene B4 on interleukin-32, interferon-? and chemokines in rats with rheumatoid arthritis. Exp Ther Med 14:2925-2930 (2017). PubMed: 28912850