Rat IgG ELISA Kit (ab157737)
Key features and details
- Sensitivity: 4.5349 ng/ml
- Range: 25 ng/ml - 800 ng/ml
- Sample type: Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
製品の概要
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製品名
Rat IgG ELISA Kit
IgG キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Overall < 10% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Overall < 10% -
サンプルの種類
Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
4.5349 ng/ml -
検出範囲
25 ng/ml - 800 ng/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Serum > 85 % - % -
ステップ
Multiple steps standard assay -
種交差性
交差種: Rat -
製品の概要
Abcam’s IgG Rat ELISA kit is an in vitro enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of IgG in rat serum and plasma samples.
In this assay the IgG present in samples reacts with the anti-IgG antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-IgG antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labeled antibodies form complexes with the previously bound IgG. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgG in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgG in the test sample. The quantity of IgG in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
Get results in 90 minutes with Rat IgG ELISA Kit (ab189578) from our SimpleStep ELISA® range.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 100X HRP-conjugated anti-rat IgG antibody 1 x 150µl 20X Wash Buffer Concentrate 1 x 50ml 5X Diluent Concentrate 1 x 50ml Chromogen Substrate Solution 1 x 12ml Rat IgG Calibrator (Lyophilized) 1 vial Rat IgG ELISA Microplate 1 unit Stop Solution 1 x 12ml -
研究分野
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細胞内局在
Secreted -
別名
- Ig gamma 1 chain C region
- Ig gamma 2 chain C region
- Ig gamma 3 chain C region
see all
関連製品
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SimpleStep ELISA kits
画像
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Sandwich ELISA - ab157737 IgG Rat ELISA Kit
Standard curve: mean of duplicates (+/- SD) with background readings subtracted
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Sandwich ELISA - ab157737 IgG Rat ELISA KitRat IgG measured in biofluids showing quantity (micrograms) per mL of tested sample
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Sandwich ELISA - ab157737 IgG Rat ELISA KitRat IgG measured in biologicals at various dilutions showing quantity (ng) per mL of tested sample
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab157737 は論文での使用が確認できていません。