Anti-Parvovirus B 19 IgM ELISA Kit (ab178651)
Key features and details
- Sensitivity: 95 %
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Indirect
- Reacts with: Human
製品の概要
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製品名
Anti-Parvovirus B 19 IgM ELISA Kit -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Pos. Serum 7 2.4% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Pos. Serum 21 3.6% -
サンプルの種類
Serum, Hep Plasma, Cit plasma -
アッセイタイプ
Indirect -
検出感度
> 95 % -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s anti-Parvovirus B 19 IgM Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgM class antibodies against Parvovirus B 19 in Human serum and plasma.
A 96-well plate has been precoated with Parvovirus B 19 antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgM conjugate is added to the wells, which binds to the immobilized Parvovirus B 19 antigens. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The intensity of yellow coloration is directly proportional to the amount of Parvovirus B 19 IgM sample captured in plate.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 ラベル 1 x 96 tests 20X Washing Solution White cap 1 x 50ml Cover Foil 1 unit IgM Sample Diluent 1 x 100ml Parvovirus B 19 (IgM) Coated Microplate (12 x 8 wells) 1 unit Parvovirus B 19 Anti-IgM HRP Conjugate 1 x 20ml Parvovirus B 19 IgM Cut-off Control 1 x 3ml Parvovirus B 19 IgM Negative Control 1 x 2ml Parvovirus B 19 IgM Positive Control 1 x 2ml Stop Solution red cap 1 x 15ml Strip holder 1 unit TMB Substrate Solution Yellow cap 1 x 15ml -
研究分野
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関連性
Parvoviruses are cubic single-stranded DNA viruses of about 18-32 nm lacking an envelope. Parvovirus B19 infects only humans, and since there are no crossreactivities between animal parvoviruses and B19, transmission between pets and humans is not possible. Parvovirus B19 is the causative agent of Erythema infectiosum, the so-called “fifth disease”, a mild rash illness that occurs most commonly in children. Infected persons are contagious during the early part of the illness before the rash appears so in adults the rate of epidemia amounts to about 60%. About 20% of adults and children who are infected with parvovirus B19 do not develop any symptoms. Persons infected with the virus, however, do develop lasting immunity that protects them against infection in the future. Parvovirus B19 infection may cause a serious illness in persons with sickle-cell disease or similar types of chronic anemia as well as in persons who have problems with their immune system (people with leukemia or cancer, who are born with immune deficiencies, who have received an organ transplant, or who have HIV infection). Occasionally (less than 5% of all pregnant women infected with parvovirus B19) serious complications may develop during pregnancy: risk of Morbus haemolyticus fetalis.
画像
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Specific antigens are coated on the 96-well plate, controls or test samples are added to the well and incubated. The wells are washed to remove any unbound Human anti-antigen antibodies (Ig). A horseradish peroxidase (HRP) labelled anti-Human Ig conjugate is added to the wells. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The intensity of yellow coloration is directly proportional to the amount of Human anti-antigen Ig captured on the plate.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab178651 は論文での使用が確認できていません。