Mouse VEGFR2 ELISA Kit (ab245725)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 28.8 pg/ml
- Range: 78.13 pg/ml - 5000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
製品の概要
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製品名
Mouse VEGFR2 ELISA Kit
VEGF Receptor 2 キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% serum 5 6.4% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% serum 3 6.9% -
サンプルの種類
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
28.8 pg/ml -
検出範囲
78.13 pg/ml - 5000 pg/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 120 113% - 124% Serum 106 102% - 110% Hep Plasma 101 90% - 107% EDTA Plasma 101 99% - 102% Cit plasma 112 106% - 117% -
全工程の試験時間
1h 30m -
ステップ
One step assay -
種交差性
交差種: Mouse -
製品の概要
Mouse VEGFR2 ELISA Kit (ab245725) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of VEGFR2 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse VEGFR2 with 28.8 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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試験プラットフォーム
Pre-coated microplate (12 x 8 well strips)
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 10X Mouse VEGFR2 Capture Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPR 1 x 6ml Mouse VEGFR2 Detector Antibody (Lyophilized) 1 vial Mouse VEGFR2 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent 50BS 1 x 20ml Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究分野
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機能
Receptor for VEGF or VEGFC. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. -
関連疾患
Defects in KDR are associated with susceptibility to hemangioma capillary infantile (HCI) [MIM:602089]. HCI are benign, highly proliferative lesions involving aberrant localized growth of capillary endothelium. They are the most common tumor of infancy, occurring in up to 10% of all births. Hemangiomas tend to appear shortly after birth and show rapid neonatal growth for up to 12 months characterized by endothelial hypercellularity and increased numbers of mast cells. This phase is followed by slow involution at a rate of about 10% per year and replacement by fibrofatty stroma. -
配列類似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
翻訳後修飾
Phosphorylated. Dephosphorylated by PTPRB. Dephosphorylated by PTPRJ at Tyr-951, Tyr-996, Tyr-1054, Tyr-1059, Tyr-1175 and Tyr-1214. -
細胞内局在
Membrane. - Information by UniProt
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別名
- CD309
- CD309 antigen
- EC 2.7.10.1
see all -
参照データベース
- Entrez Gene: 16542 Mouse
- SwissProt: P35918 Mouse
- Unigene: 285 Mouse
関連製品
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Alternative Versions
画像
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Other - Mouse VEGF Receptor 2 ELISA Kit (ab245725)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Mouse VEGF Receptor 2 Standard Curve Comparison.Standard Curve comparison between mouse VEGF Receptor 2 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
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Example of mouse VEGF Receptor 2 standard curve in Sample Diluent 50BS.The VEGF Receptor 2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native VEGF Receptor 2 in mouse serum and plasma samples.The concentrations of VEGF Receptor 2 were measured in duplicates, interpolated from the VEGF Receptor 2 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 10%, plasma (citrate) 10%, plasma (heparin) 10%, and plasma (EDTA) 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean VEGF Receptor 2 concentration was determined to be 38.9 ng/mL in serum, 27.9 ng/mL in plasma (citrate), 42.1 ng/mL in plasma (heparin), and 24.1 ng/mL in plasma (EDTA).
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Interpolated concentrations of native VEGF Receptor 2 in mouse kidney tissue culture supernatant samples.The concentrations of VEGF Receptor 2 were measured in duplicates, interpolated from the VEGF Receptor 2 standard curves and corrected for sample dilution. Undiluted samples are as follows: kidney supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean VEGF Receptor 2 concentration was determined to be 1,291.7 pg/mL in mouse kidney supernatant.
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Sandwich ELISA - Mouse VEGF Receptor 2 ELISA Kit (ab245725)To learn more about the advantages of recombinant antibodies see here. -
Sandwich ELISA - Mouse VEGFR2 ELISA Kit (ab245725)To learn more about the advantages of SimpleStep ELISA® kits see here.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab245725 は論文での使用が確認できていません。