Mouse Prothrombin ELISA Kit (ab157526)
Key features and details
- Sensitivity: 0.386 ng/ml
- Range: 1 ng/ml - 500 ng/ml
- Sample type: Cell culture media, Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
製品の概要
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製品名
Mouse Prothrombin ELISA Kit
Prothrombin キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% 1 20 2.48ng/ml 0.148 5.96% 2 20 11.9ng/ml 0.935 7.86% 3 20 41.8ng/ml 3.83 9.16% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% 1 10 2.24ng/ml 0.159 7.11% 2 10 12.9ng/ml 1.27 9.85% 3 10 47.2ng/ml 2.68 5.68% -
サンプルの種類
Serum, Plasma, Tissue Extracts, Cell culture media -
アッセイタイプ
Sandwich (quantitative) -
検出感度
0.386 ng/ml -
検出範囲
1 ng/ml - 500 ng/ml -
添加回収試験
105.85 %
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ステップ
Multiple steps standard assay -
種交差性
交差種: Mouse -
製品の概要
Abcam’s Prothrombin ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative determination of prothrombin in Mouse plasma, serum, tissue extracts and cell culture media. The assay will not detect Mouse Thrombin.
Mouse Prothrombin will bind to the capture antibody coated on the microtiter plate. Thrombin and thrombin-antithrombin complex will not react with the plate. After appropriate washing steps, biotinylated primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with horseradish peroxidase conjugated streptavidin. TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of prothrombin. The amount of color development is directly proportional to the concentration of Prothrombin in the sample.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 10X Wash Buffer 1 x 50ml Anti-mouse Prothrombin primary antibody 1 vial Anti-prothrombin coated Microtiter Plate (8 x 12 wells) 1 unit HRP-conjugated Streptavidin 1 vial Prothrombin Standard 1 vial TMB Substrate Solution 1 x 10ml -
研究分野
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機能
Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing. -
組織特異性
Expressed by the liver and secreted in plasma. -
関連疾患
Defects in F2 are the cause of factor II deficiency (FA2D) [MIM:613679]. It is a very rare blood coagulation disorder characterized by mucocutaneous bleeding symptoms. The severity of the bleeding manifestations correlates with blood factor II levels.
Genetic variations in F2 may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors.
Defects in F2 are a cause of susceptibility to thrombosis (THR) [MIM:188050]. It is a multifactorial disorder of hemostasis characterized by abnormal platelet aggregation in response to various agents and recurrent thrombi formation. Note=A common genetic variation in the 3-prime untranslated region of the prothrombin gene is associated with elevated plasma prothrombin levels and an increased risk of venous thrombosis. -
配列類似性
Belongs to the peptidase S1 family.
Contains 1 Gla (gamma-carboxy-glutamate) domain.
Contains 2 kringle domains.
Contains 1 peptidase S1 domain. -
翻訳後修飾
The gamma-carboxyglutamyl residues, which bind calcium ions, result from the carboxylation of glutamyl residues by a microsomal enzyme, the vitamin K-dependent carboxylase. The modified residues are necessary for the calcium-dependent interaction with a negatively charged phospholipid surface, which is essential for the conversion of prothrombin to thrombin. -
細胞内局在
Secreted > extracellular space. - Information by UniProt
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別名
- Coagulation factor II
- coagulation factor II (thrombin)
- F2
see all -
参照データベース
- Entrez Gene: 14061 Mouse
- SwissProt: P19221 Mouse
- Unigene: 89048 Mouse
画像
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Example Standard Curve for ab157526
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab157526 は 2 報の論文で使用されています。
- Lei J et al. Dabigatran activates inflammation resolution by promoting fibrinogen-like protein 2 shedding and RvD5n-3 DPA production. Theranostics 11:4251-4261 (2021). PubMed: 33754059
- Ezquer F et al. Administration of multipotent mesenchymal stromal cells restores liver regeneration and improves liver function in obese mice with hepatic steatosis after partial hepatectomy. Stem Cell Res Ther 8:20 (2017). Mouse . PubMed: 28129776