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Mouse Myoglobin ELISA Kit (ab157722)

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Standard Curve

    Key features and details

    • Sensitivity: 4.48 ng/ml
    • Range: 9.375 ng/ml - 300 ng/ml
    • Sample type: Plasma, Serum
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Mouse

    製品の概要

    • 製品名

      Mouse Myoglobin ELISA Kit
      Myoglobin キット 製品一覧

    • 検出方法

      Colorimetric

    • 再現性

      Intra-Assay（同時再現性）
      サンプル N 平均値 SD CV%
      Overall < 10%
      Inter-Assay（日差再現性）
      サンプル N 平均値 SD CV%
      Overall < 10%

    • サンプルの種類

      Serum, Plasma

    • アッセイタイプ

      Sandwich (quantitative)

    • 検出感度

      4.48 ng/ml

    • 検出範囲

      9.375 ng/ml - 300 ng/ml

    • 添加回収試験

      特定サンプルでの回収試験
      サンプルの種類 平均 % 測定範囲
      Serum > 85 % - %

    • ステップ

      Multiple steps standard assay

    • 種交差性

      交差種: Mouse

    • 製品の概要

      Abcam’s Myoglobin Mouse ELISA kit is an in vitro enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of Myoglobin in mouse samples. 


      In this assay the Myoglobin present in samples reacts with the anti-Myoglobin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Myoglobin antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Myoglobin. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Myoglobin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Myoglobin in the test sample. The quantity of Myoglobin in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.


      Get better reproducibility in only 90 minutes with Mouse Myoglobin ELISA Kit (ab210965) from our SimpleStep ELISA® range.

    • 試験プラットフォーム

      Microplate

    製品の特性

    関連製品

    画像

    • Standard Curve
      Standard Curve
      Representative standard curve using ab157722 Myoglobin Mouse ELISA Kit

    参考文献 (1)

    ab157722 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab157722 は 1 報の論文で使用されています。

    • Warfel JD  et al. A low fat diet ameliorates pathology but retains beneficial effects associated with CPT1b knockout in skeletal muscle. PLoS One 12:e0188850 (2017). ELISA ; Mouse . PubMed: 29240830

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