Mouse IgG2c ELISA Kit (ab157720)
Key features and details
- Sensitivity: 0.38 ng/ml
- Range: 3.13 ng/ml - 200 ng/ml
- Sample type: Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
製品の概要
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製品名
Mouse IgG2c ELISA Kit -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Overall < 10% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Overall < 10% -
サンプルの種類
Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
0.38 ng/ml -
検出範囲
3.13 ng/ml - 200 ng/ml -
添加回収試験
> 85 %
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Serum > 85 % - % -
ステップ
Multiple steps standard assay -
種交差性
交差種: Mouse -
製品の概要
Abcam’s IgG2c Mouse ELISA kit is an in vitro enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of IgG2c in mouse samples.
In this assay the IgG2c present in samples reacts with the anti-IgG2c antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-IgG2c antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound IgG2c. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgG2c in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgG2c in the test sample. The quantity of IgG2c in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 100X HRP-conjugated anti-mouse IgG2c antibody 1 x 150µl 20X Wash Buffer Concentrate 1 x 50ml 5X Diluent Concentrate 1 x 50ml Chromogen Substrate Solution 1 x 12ml Mouse IgG2c Calibrator (lyophilized) 1 vial Mouse IgG2c ELISA Microplate 1 unit Stop Solution 1 x 12ml -
研究分野
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関連性
There are four IgG subclasses (IgG1, 2, 3 and 4) in Humans, named in order of their abundance in serum (IgG1 being the most abundant). IgG2 is the only IgG subclass which passes through the placenta at a level generally lower than that found in the mother. A deficiency of IgG2 indicates a poor antibody response to bacterial polysaccharides and can lead to increased susceptibility to infections caused by encapsulated bacteria. -
別名
- Ig gamma-2 chain C region
画像
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Standard CurveRepresentative standard curve using ab157720 IgG2c Mouse ELISA Kit
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab157720 は 3 報の論文で使用されています。
- Hao L et al. Combinational PRR Agonists in Liposomal Adjuvant Enhances Immunogenicity and Protective Efficacy in a Tuberculosis Subunit Vaccine. Front Immunol 11:575504 (2020). PubMed: 33117374
- Ullah N et al. Differential Immunogenicity and Protective Efficacy Elicited by MTO- and DMT-Adjuvanted CMFO Subunit Vaccines against Mycobacterium tuberculosis Infection. J Immunol Res 2020:2083793 (2020). PubMed: 32953889
- Ma J et al. A Multistage Subunit Vaccine Effectively Protects Mice Against Primary Progressive Tuberculosis, Latency and Reactivation. EBioMedicine 22:143-154 (2017). PubMed: 28711483