Mouse IGFBP7 ELISA Kit (ab245712)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 6.03 pg/ml
- Range: 39.06 pg/ml - 2500 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Serum, Tissue Extracts, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
製品の概要
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製品名
Mouse IGFBP7 ELISA Kit
IGFBP7 キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% serum 8 2.6% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% serum 3 11.2% -
サンプルの種類
Cell culture supernatant, Urine, Serum, Tissue Extracts, EDTA Plasma, Cit plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
6.03 pg/ml -
検出範囲
39.06 pg/ml - 2500 pg/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 92 83% - 100% Urine 102 100% - 104% Serum 106 103% - 111% Tissue Extracts 99 97% - 100% EDTA Plasma 97 92% - 104% Cit plasma 104 100% - 108% -
全工程の試験時間
1h 30m -
ステップ
One step assay -
種交差性
交差種: Mouse
非交差種: Cow -
製品の概要
Mouse IGFBP7 ELISA Kit (ab245712) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IGFBP7 protein in cell culture supernatant, cit plasma, edta plasma, serum, tissue extracts, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse IGFBP7 with 6.03 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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特記事項
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
試験プラットフォーム
Pre-coated microplate (12 x 8 well strips)
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 10X Mouse IGFBP7 Capture Antibody 1 x 600µl 10X Mouse IGFBP7 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BR 1 x 6ml Mouse IGFBP7 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究分野
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機能
Binds IGF-I and IGF-II with a relatively low affinity. Stimulates prostacyclin (PGI2) production. Stimulates cell adhesion. -
配列類似性
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 IGFBP N-terminal domain.
Contains 1 Kazal-like domain. -
翻訳後修飾
N-glycosylated. -
細胞内局在
Secreted. - Information by UniProt
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別名
- AGM
- Angiomodulin
- FSTL2
see all -
参照データベース
- Entrez Gene: 29817 Mouse
- SwissProt: Q61581 Mouse
- Unigene: 233470 Mouse
関連製品
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Alternative Versions
画像
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Other - Mouse IGFBP7 ELISA Kit (ab245712)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse IGFBP7 standard curve in Sample Diluent NS.The IGFBP7 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed. -
Example of mouse IGFBP7 standard curve in 1X Cell Extraction Buffer PTR.The IGFBP7 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed. -
Interpolated concentrations of native IGFBP7 in mouse serum, plasma, urine, and cell culture supernatant samples.The concentrations of IGFBP7 were measured in duplicates, interpolated from the IGFBP7 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1%, plasma (citrate) 1%, plasma (EDTA) 1%, urine 1%, and 3T3-L1 cell culture supernatant 6.25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGFBP7 concentration was determined to be 87.42 ng/mL in serum, 74.99 ng/mL in plasma (citrate), 85.41 ng/mL in plasma (EDTA) 28.69 ng/mL in urine, and 24.46 ng/mL in 3T3-L1 cell culture supernatant. -
Interpolated concentrations of native IGFBP7 in mouse lung extract (25 ug/mL load) and spleen extract (50 ug/mL load).The concentrations of IGFBP7 were measured in duplicate and interpolated from the IGFBP7 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGFBP7 concentration was determined to be 2039.98 pg/mL in lung extract and 2422.48 pg/mL in spleen extract. -
Sandwich ELISA - Mouse IGFBP7 ELISA Kit (ab245712)To learn more about the advantages of recombinant antibodies see here.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab245712 は論文での使用が確認できていません。