Mouse CXCL1 ELISA Kit (GRO alpha) (ab100717)
Key features and details
- Sensitivity: 1 pg/ml
- Range: 0.614 pg/ml - 150 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
製品の概要
-
製品名
Mouse CXCL1 ELISA Kit (GRO alpha)
CXCL1/GRO alpha キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 1 pg/ml -
検出範囲
0.614 pg/ml - 150 pg/ml -
添加回収試験
100 %
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 123.5 115% - 139% Serum 89.3 78% - 97% Plasma 101.4 88% - 113% -
ステップ
Multiple steps standard assay -
種交差性
交差種: Mouse -
製品の概要
Abcam’s Mouse CXCL1 ELISA (Enzyme-Linked Immunosorbent Assay) kit (GRO alpha) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse CXCL1 (GRO alpha) in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse CXCL1 coated on a 96-well plate. Standards and samples are pipetted into the wells and CXCL1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse CXCL1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CXCL1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
-
試験プラットフォーム
Microplate
製品の特性
-
保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent 1 x 15ml Biotinylated anti-mouse GRO alpha 2 vials GRO alpha Microplate (12 x 8 wells) 1 unit Recombinant mouse GRO alpha Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究分野
-
機能
Has chemotactic activity for neutrophils. May play a role in inflammation and exerts its effects on endothelial cells in an autocrine fashion. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity. -
配列類似性
Belongs to the intercrine alpha (chemokine CxC) family. -
翻訳後修飾
N-terminal processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) are produced by proteolytic cleavage after secretion from peripheral blood monocytes. -
細胞内局在
Secreted. - Information by UniProt
-
別名
- C-X-C motif chemokine 1
- chemokine (C-X-C motif) ligand 1
- Chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha)
see all -
参照データベース
- Entrez Gene: 14825 Mouse
- SwissProt: P12850 Mouse
画像
-
Typical standard curve
Representative standard curve using ab100717
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab100717 は論文での使用が確認できていません。