Mouse CD80 ELISA Kit (ab245721)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 2 pg/ml
- Range: 6.25 pg/ml - 400 pg/ml
- Sample type: Cell culture extracts, Cell culture supernatant, Cit plasma, Hep Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
製品の概要
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製品名
Mouse CD80 ELISA Kit
CD80 キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% serum 5 5.6% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% serum 3 5.7% -
サンプルの種類
Cell culture supernatant, Serum, Cell culture extracts, Tissue Extracts, Hep Plasma, Cit plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
2 pg/ml -
検出範囲
6.25 pg/ml - 400 pg/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 106 100% - 113% Serum 101 91% - 110% Cell culture extracts 99 88% - 106% Hep Plasma 111 108% - 115% Cit plasma 107 99% - 117% -
全工程の試験時間
1h 30m -
ステップ
One step assay -
種交差性
交差種: Mouse -
製品の概要
Mouse CD80 ELISA Kit (ab245721) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CD80 protein in cell culture extracts, cell culture supernatant, cit plasma, hep plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse CD80 with 2 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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特記事項
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
試験プラットフォーム
Pre-coated microplate (12 x 8 well strips)
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 10X Mouse CD80 Capture Antibody 1 x 600µl 10X Mouse CD80 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 5BR 1 x 6ml Mouse CD80 Lyophilized recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究分野
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機能
Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor. -
組織特異性
Expressed on activated B-cells, macrophages and dendritic cells. -
配列類似性
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
細胞内局在
Membrane. - Information by UniProt
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別名
- Activation B7-1 antigen
- B lymphocyte activation antigen B7
- B7
see all -
参照データベース
- Entrez Gene: 12519 Mouse
- SwissProt: Q00609 Mouse
- Unigene: 89474 Mouse
関連製品
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Alternative Versions
画像
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Other - Mouse CD80 ELISA Kit (ab245721)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse CD80 standard curve in Sample Diluent NS.The CD80 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed. -
Example of mouse CD80 standard curve in 1X Cell Extraction Buffer PTR.The CD80 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed. -
Interpolated concentrations of native CD80 in mouse serum, plasma, and cell culture supernatant samples.The concentrations of CD80 were measured in duplicates, interpolated from the CD80 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (citrate) 50%, plasma (heparin) 25%, and spleen supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD80 concentration was determined to be 1,505.1 pg/mL in serum, 829.0 pg/mL in plasma (citrate), 1,487.5 pg/mL in plasma (heparin), and 228.8 pg/ml in spleen supernatant. -
Interpolated concentrations of native CD80 in mouse C2C12 cell extract based on a 10 µg/mL extract load.The concentrations of CD80 were measured in duplicate and interpolated from the CD80 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD80 concentration was determined to be 314.7 pg/mL in C2C12 cell extract. -
Sandwich ELISA - Mouse CD80 ELISA Kit (ab245721)To learn more about the advantages of recombinant antibodies see here. -
Sandwich ELISA - Mouse CD80 ELISA Kit (ab245721)To learn more about the advantages of SimpleStep ELISA® kits see here.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab245721 は論文での使用が確認できていません。