Monkey IL-6 ELISA Kit (ab242233)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 3.2 pg/ml
- Range: 12.5 pg/ml - 800 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Monkey, Cynomolgus monkey, Rhesus monkey
製品の概要
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製品名
Monkey IL-6 ELISA Kit
IL-6 キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% cell culture 8 5.5% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% cell culture 3 5% -
サンプルの種類
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
3.2 pg/ml -
検出範囲
12.5 pg/ml - 800 pg/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 90 86% - 94% Serum 76 75% - 77% Cell culture media 101 95% - 109% Hep Plasma 103 94% - 114% EDTA Plasma 85 78% - 92% Cit plasma 78 76% - 81% -
全工程の試験時間
1h 30m -
ステップ
One step assay -
種交差性
交差種: Monkey, Cynomolgus monkey, Rhesus monkey -
製品の概要
Monkey IL-6 ELISA Kit (ab242233) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-6 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Monkey IL-6 with 3.2 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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試験プラットフォーム
Pre-coated microplate (12 x 8 well strips)
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 10 x 96 tests 1 x 384 tests 10X Monkey IL-6 Capture Antibody 1 x 600µl 1 x 6000µl 1 x 600µl 10X Monkey IL-6 Detector Antibody 1 x 600µl 1 x 6000µl 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 1 x 200ml 1 x 20ml 384 well CaptSure™ microplates 0 x 0 unit 0 x 0 unit 1 unit Antibody Diluent 5BI 1 x 6ml 10 x 6ml 1 x 6ml Monkey IL-6 Lyophilized recombinant Protein 2 vials 2 x 10 vials 2 vials Plate Seals 1 unit 1 x 10 units 1 unit Sample Diluent NS (ab193972) 1 x 50ml 2 x 250ml 2 x 20ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit 1 x 10 units 0 x 0 unit Stop Solution 1 x 12ml 1 x 120ml 2 x 12ml TMB Development Solution 1 x 12ml 1 x 120ml 2 x 12ml -
研究分野
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機能
Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. -
関連疾患
Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men. -
配列類似性
Belongs to the IL-6 superfamily. -
翻訳後修飾
N- and O-glycosylated. -
細胞内局在
Secreted. - Information by UniProt
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別名
- Interleukin BSF 2
- B cell differentiation factor
- B cell stimulatory factor 2
see all -
参照データベース
- SwissProt: P51494 Rhesus monkey
関連製品
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Alternative Versions
画像
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Example of monkey IL-6 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
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The IL-6 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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PBMCs were cultured in the presence (stimulated) or absence of 50 ng/mL PMA + 1 µg/mL Ionomycin for 24 hours. The concentrations of IL-6 were measured in duplicates, interpolated from the IL-6 standard curves and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-6 concentration was determined to be 696.3 pg/mL in neat stimulated PBMC cell culture supernatant, and undetectable in unstimulated PBMC cell culture supernatant, and naive cell culture media controls (not shown).
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PBMCs were cultured in the presence or absence of 50 ng/mL PMA and 1 µg/mL Ionomycin for 24 hours. The concentrations of IL-6 were measured in duplicates, interpolated from the IL-6 standard curves and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-6 concentration was determined to be 696.3 pg/mL in neat stimulated rhesus PBMC supernatant and 907.3 pg/mL in neat stimulated cynomolgus PBMC supernatant. Values for unstimulated supernatants were undetectable in both rhesus and cynomolgus samples (not shown).
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SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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To learn more about the advantages of recombinant antibodies see here.
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To learn more about the advantages of SimpleStep ELISA® kits see here.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab242233 は 2 報の論文で使用されています。
- Qu L et al. Circular RNA vaccines against SARS-CoV-2 and emerging variants. Cell 185:1728-1744.e16 (2022). PubMed: 35460644
- Cheng Y et al. The Effect of Sevoflurane Anesthesia on the Biomarkers of Neural Injury in the Prefrontal Cortex of Aged Marmosets. Front Aging Neurosci 14:918640 (2022). PubMed: 35847680