Key features and details
- Sensitivity: 2.5 mU/ml
- Range: 2.5 mU/ml - 80 mU/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品名Human Von Willebrand Factor ELISA Kit (with plasma controls)
Von Willebrand Factor キット 製品一覧
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Overall 5% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Overall 7.1%
サンプルの種類Cell culture supernatant, Serum, Plasma
検出感度> 2.5 mU/ml
検出範囲2.5 mU/ml - 80 mU/ml
Abcam’s Von Willebrand Factor (VWF) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Von Willebrand Factor levels in cell culture supernatants, plasma and serum.
A Von Willebrand Factor specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Von Willebrand Factor specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Von Willebrand Factor captured in plate.
Get higher sensitivity in only 90 minutes with Human Von Willebrand Factor ELISA Kit (ab189571) from our SimpleStep ELISA® range.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
保存方法Store at -20°C. Please refer to protocols.
内容 1 x 96 tests 100X Biotinylated Human Von Williebrand Factor Antibody 1 x 60µl 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml Chromogen Substrate 1 x 7ml Low control (Depleted Human Plasma) 1 vial Positive control (Reference Plasma Control) 1 vial Sealing Tapes 3 units Stop Solution 1 x 11ml Von Williebrand Factor Microplate (12 x 8 well strips) 1 x 96 tests Von Williebrand Factor Standard 1 vial
機能Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma.
関連疾患Defects in VWF are the cause of von Willebrand disease (VWD) [MIM:277480]. VWD defines a group of hemorrhagic disorders in which the von Willebrand factor is either quantitatively or qualitatively abnormal resulting in altered platelet function. Symptoms vary depending on severity and disease type but may include prolonged bleeding time, deficiency of factor VIII and impaired platelet adhesion. Type I von Willebrand disease is the most common form and is characterized by partial quantitative plasmatic deficiency of an otherwise structurally and functionally normal Willebrand factor; type II is associated with a qualitative deficiency and functional anomalies of the Willebrand factor; type III is the most severe form and is characterized by total or near-total absence of Willebrand factor in the plasma and cellular compartments, also leading to a profound deficiency of plasmatic factor VIII.
配列類似性Contains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 4 TIL (trypsin inhibitory-like) domains.
Contains 3 VWFA domains.
Contains 3 VWFC domains.
Contains 4 VWFD domains.
ドメインThe von Willebrand antigen 2 is required for multimerization of vWF and for its targeting to storage granules.
翻訳後修飾All cysteine residues are involved in intrachain or interchain disulfide bonds.
N- and O-glycosylated.
細胞内局在Secreted. Secreted > extracellular space > extracellular matrix. Localized to storage granules.
- Information by UniProt
- Coagulation factor VIII
- Coagulation factor VIII VWF
ab168548 は 3 報の論文で使用されています。
- D'Alessandro A et al. Biological and Clinical Factors contributing to the Metabolic Heterogeneity of Hospitalized Patients with and without COVID-19. Res Sq N/A:N/A (2021). PubMed: 34013258
- D'Alessandro A et al. Biological and Clinical Factors Contributing to the Metabolic Heterogeneity of Hospitalized Patients with and without COVID-19. Cells 10:N/A (2021). PubMed: 34571942
- Thangaraju K et al. The Impact of Age and BMI on the VWF/ADAMTS13 Axis and Simultaneous Thrombin and Plasmin Generation in Hospitalized COVID-19 Patients. Front Med (Lausanne) 8:817305 (2021). PubMed: 35087853