Human Prorenin ELISA Kit (ab157525)
Key features and details
- Sensitivity: 0.013 ng/ml
- Range: 0.02 ng/ml - 10 ng/ml
- Sample type: Plasma
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human Prorenin ELISA Kit
Prorenin キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% 1 20 0.345ng/ml 0.02 5.74% 2 20 0.464ng/ml 0.017 3.61% 3 20 6.22ng/ml 0.237 3.81% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% 1 10 0.342ng/ml 0.045 13.2% 2 10 0.439ng/ml 0.056 12.6% 3 10 6.16ng/ml 0.353 5.72% -
サンプルの種類
Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
0.013 ng/ml -
検出範囲
0.02 ng/ml - 10 ng/ml -
添加回収試験
99.75 %
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ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s Prorenin ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for is for the quantitative determination of Prorenin in Human plasma. Active renin will not be detected by this assay. Prorenin is measured directly by ELISA without pre-treatment of samples or conversion to renin.
Human Prorenin will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, anti-Human Prorenin primary antibody binds to the captured protein. Only Prorenin and not active renin will be detected by the primary antibody. Excess antibody is washed away and bound primary antibody is then reacted with the secondary antibody conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of Prorenin. The amount of color development is directly proportional to the concentration of Prorenin in the sample.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Please refer to protocols. -
内容 1 x 96 tests 10X Wash Buffer 1 x 50ml Anti-Human Prorenin antibody coated plates 1 unit Anti-Human Prorenin Primary Antibody 1 vial Anti-mouse HRP Secondary Antibody 1 vial Human Prorenin Negative Control (0 ng) 2 vials Human Prorenin Positive Control (20 ng) 1 vial TMB Substrate Solution 1 x 10ml -
研究分野
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機能
Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney. -
関連疾患
Defects in REN are a cause of renal tubular dysgenesis (RTD) [MIM:267430]. RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype).
Defects in REN are the cause of familial juvenile hyperuricemic nephropathy type 2 (HNFJ2) [MIM:613092]. It is a renal disease characterized by juvenile onset of hyperuricemia, slowly progressive renal failure and anemia. -
配列類似性
Belongs to the peptidase A1 family. -
細胞内局在
Secreted. Membrane. Associated to membranes via binding to ATP6AP2. - Information by UniProt
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別名
- Angiotensin forming enzyme
- Angiotensinogenase
- FLJ10761
see all -
参照データベース
- Entrez Gene: 5972 Human
- Omim: 179820 Human
- SwissProt: P00797 Human
- Unigene: 3210 Human
画像
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Typical Standard Cuve for ab157525 Prorenin Human ELISA
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab157525 は論文での使用が確認できていません。