Human Prekallikrein and Kallikrein ELISA Kit (ab171015)
Key features and details
- Sensitivity: 0.54 ng/ml
- Range: 5 ng/ml - 40 ng/ml
- Sample type: Cell culture supernatant, Cell Lysate, Cerebral Spinal Fluid, Milk, Plasma, Saliva, Serum, Tissue
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human Prekallikrein and Kallikrein ELISA Kit
Plasma Kallikrein 1B キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Overall 5.9% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Overall 9.8% -
サンプルの種類
Cell culture supernatant, Saliva, Milk, Serum, Plasma, Tissue, Cell Lysate, Cerebral Spinal Fluid -
アッセイタイプ
Sandwich (quantitative) -
検出感度
0.54 ng/ml -
検出範囲
5 ng/ml - 40 ng/ml -
添加回収試験
97 %
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全工程の試験時間
4h 00m -
種交差性
交差種: Human -
製品の概要
Abcam’s Prekallikrein and Kallikrein Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Prekallikrein and Kallikrein levels in plasma, serum, saliva, milk, cerebrospinal fluid, cell culture, and cell lysate samples.
A Prekallikrein specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Prekallikrein specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Prekallikrein and Kallikrein captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human Prekallikrein Antibody 1 x 120µl Chromogen Substrate 1 x 7ml Prekallikrein Microplate (12 x 8 well strips) 1 unit Prekallikrein Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 11ml -
研究分野
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機能
The enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin. -
関連疾患
Defects in KLKB1 are the cause of prekallikrein deficiency (PKK deficiency) [MIM:612423]; also known as Fletcher factor deficiency. This disorder is a blood coagulation defect. -
配列類似性
Belongs to the peptidase S1 family. Plasma kallikrein subfamily.
Contains 4 apple domains.
Contains 1 peptidase S1 domain. -
細胞内局在
Secreted. - Information by UniProt
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別名
- Fletcher factor
- kallikrein B plasma
- Kallikrein B plasma (Fletcher factor) 1
see all -
参照データベース
- Entrez Gene: 3818 Human
- Omim: 229000 Human
- SwissProt: P03952 Human
- Unigene: 237642 Human
画像
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Typical Standard Curve
Representative Standard Curve using ab171015
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab171015 は 3 報の論文で使用されています。
- Scheffel J et al. Cold-induced urticarial autoinflammatory syndrome related to factor XII activation. Nat Commun 11:179 (2020). PubMed: 31924766
- Zitur LJ et al. Brain Death Enhances Activation of the Innate Immune System and Leads to Reduced Renal Metabolic Gene Expression. Transplantation 103:1821-1833 (2019). PubMed: 30964836
- Harbaum L et al. Reduced plasma levels of small HDL particles transporting fibrinolytic proteins in pulmonary arterial hypertension. Thorax 74:380-389 (2019). PubMed: 30478197