Human PAI1 ELISA Kit (ab157528)
Key features and details
- Sensitivity: 0.11 U/ml
- Range: 0.125 U/ml - 100 U/ml
- Sample type: Plasma
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human PAI1 ELISA Kit
PAI1 キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% 1 20 2.8U/ml 0.257 9.18% 2 20 6.82U/ml 0.323 4.74% 3 20 29.6U/ml 2.57 8.68% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% 1 10 4.2U/ml 0.399 9.52% 2 10 8.07U/ml 0.763 9.46% 3 10 26.8U/ml 2.1 7.85% -
サンプルの種類
Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
= 0.11 U/ml -
検出範囲
0.125 U/ml - 100 U/ml -
添加回収試験
96.45 %
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ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s PAI1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for is for the quantitative determination of active plasminogen activator inhibitor type 1 in Human plasma.
PAI1 is involved in the regulation of the blood fibrinolytic system. Increased plasma levels of PAI1 are implicated in the impairment of fibrinolytic function and may be associated with thrombotic diseases. Levels of PAI1 increase with age and are elevated in conditions such as normal pregnancy and sepsis.
Functionally active PAI1 present in plasma reacts with urokinase coated and dried on a microtiter plate. Latent or complexed PAI1 will not bind to the plate or be detected. Unbound PAI1 samples are aspirated and an anti-PAI1 primary antibody is added. Excess primary antibody is then aspirated. The bound antibody, which is proportional to the original active PAI1 present in the samples, is then reacted with the horseradish peroxidase conjugated secondary antibody. Following an additional washing step, TMB substrate solution is then used for color development at 450nm. The amount of color development is directly proportional to the concentration of active PAI1 in the sample.
Get results in 90 minutes with Human PAI1 ELISA Kit (SERPINE1) (ab184863) from our SimpleStep ELISA® range.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 10X Wash Buffer 1 x 50ml Anti-Human PAI1 Primary Antibody 1 vial Assay Diluent 1 x 10ml HRP secondary antibody 1 vial Human PAI1 high activity standard (260U) 1 vial Human PAI1 zero unit activity standard (0 U) 2 vials TMB Substrate Solution 1 x 10ml uPA Antibody pre-coated 96 well microplate (12 x 8 well strips) 1 unit -
研究分野
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機能
This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis. -
組織特異性
Found in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells. -
関連疾患
Defects in SERPINE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1D) [MIM:613329]. It is a hematologic disorder characterized by increased bleeding after trauma, injury, or surgery. Affected females have menorrhagia. The bleeding defect is due to increased fibrinolysis of fibrin blood clots due to deficiency of plasminogen activator inhibitor-1, which inhibits tissue and urinary activators of plasminogen.
Note=High concentrations of SERPINE1 seem to contribute to the development of venous but not arterial occlusions. -
配列類似性
Belongs to the serpin family. -
翻訳後修飾
Inactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg-
-Met-370 bond. -
細胞内局在
Secreted. - Information by UniProt
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別名
- Clade E
- Endothelial plasminogen activator inhibitor
- Nexin
see all -
参照データベース
- Entrez Gene: 5054 Human
- Omim: 173360 Human
- SwissProt: P05121 Human
- Unigene: 414795 Human
- Unigene: 713079 Human
画像
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Typical Standard Cuve for ab157528 PAI-1 Human ELISA Linear Range
Typical Standard Cuve for ab157528 PAI-1 Human ELISA Linear Range
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Typical Standard Cuve for ab157528 PAI-1 Human ELISA Full rangeTypical Standard Cuve for ab157528 PAI-1 Human ELISA Full range
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab157528 は論文での使用が確認できていません。