Human MyD88 ELISA Kit (ab171341)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 156 pg/ml - 10000 pg/ml
- Sample type: Cell culture supernatant, Cell Lysate, EDTA Plasma, Hep Plasma
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human MyD88 ELISA Kit
MyD88 キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Cell culture supernatant, Cell Lysate, Hep Plasma, EDTA Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 10 pg/ml -
検出範囲
156 pg/ml - 10000 pg/ml -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s Human MyD88 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human MyD88 in cell culture supernatants.
A MyD88 specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for MyD88 is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human MyD88 amount of sample captured in plate.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 ラベル 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml Antibody Diluent Buffer Green Cap 1 x 12ml Anti-Human MyD88 Antibody Microplate (12 x 8 wells) 1 x 96 tests Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl Biotinylated anti-Human MyD88 antibody 1 x 100µl Lyophilized recombinant Human MyD88 standard 2 x 10ng Plate Seal 1 x 4 units Sample Diluent Buffer Green Cap 1 x 30ml TMB Color Developing Agent Amber Bottle 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml -
研究分野
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機能
Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Increases IL-8 transcription. Involved in IL-18-mediated signaling pathway. -
組織特異性
Ubiquitous. -
関連疾患
Defects in MYD88 are the cause of MYD88 deficiency (MYD88D) [MIM:612260]; also known as recurrent pyogenic bacterial infections due to MYD88 deficiency. Patients suffer from autosomal recessive, life-threatening, often recurrent pyogenic bacterial infections, including invasive pneumococcal disease, and die between 1 and 11 months of age. Surviving patients are otherwise healthy, with normal resistance to other microbes, and their clinical status improved with age. -
配列類似性
Contains 1 death domain.
Contains 1 TIR domain. -
ドメイン
The intermediate domain (ID) is required for the phosphorylation and activation of IRAK. -
細胞内局在
Cytoplasm. - Information by UniProt
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別名
- Mutant myeloid differentiation primary response 88
- MYD 88
- Myd88
see all -
参照データベース
- Entrez Gene: 4615 Human
- Omim: 602170 Human
- SwissProt: Q99836 Human
- Unigene: 82116 Human
画像
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Typical standard curve
Representative standard curve using ab171341
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab171341 は 4 報の論文で使用されています。
- Cirmi S et al. Oleacein Attenuates Lipopolysaccharide-Induced Inflammation in THP-1-Derived Macrophages by the Inhibition of TLR4/MyD88/NF-κB Pathway. Int J Mol Sci 23:N/A (2022). PubMed: 35163130
- Zhang X et al. Involvement of TLR2/4-MyD88-NF-?B signaling pathway in the pathogenesis of intracranial aneurysm. Mol Med Rep 23:N/A (2021). PubMed: 33655339
- Quagliariello V et al. Polydatin Reduces Cardiotoxicity and Enhances the Anticancer Effects of Sunitinib by Decreasing Pro-Oxidative Stress, Pro-Inflammatory Cytokines, and NLRP3 Inflammasome Expression. Front Oncol 11:680758 (2021). PubMed: 34178667
- Quagliariello V et al. NLRP3 as Putative Marker of Ipilimumab-Induced Cardiotoxicity in the Presence of Hyperglycemia in Estrogen-Responsive and Triple-Negative Breast Cancer Cells. Int J Mol Sci 21:N/A (2020). PubMed: 33096896