Human MIA ELISA Kit (ab171340)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 15.6 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, EDTA Plasma, Hep Plasma, Saliva, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human MIA ELISA Kit -
検出方法
Colorimetric -
サンプルの種類
Cell culture supernatant, Saliva, Serum, Hep Plasma, EDTA Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 10 pg/ml -
検出範囲
15.6 pg/ml - 1000 pg/ml -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s Human MIA in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human MIA in cell culture supernatants, serum and plasma (heparin, EDTA) and saliva.
A MIA specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for MIA is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human MIA amount of sample captured in plate.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 ラベル 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml Antibody Diluent Buffer Green Cap 1 x 12ml Anti-Human MIA Antibody Microplate (12 x 8 wells) 1 x 96 tests Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl Biotinylated anti-Human MIA antibody 1 x 100µl Lyophilized recombinant Human MIA standard 2 x 1ng Plate Seal 4 units Sample Diluent Buffer Green Cap 1 x 30ml TMB Color Developing Agent Amber Bottle 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml -
研究分野
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機能
Elicits growth inhibition on melanoma cells in vitro as well as some other neuroectodermal tumors, including gliomas. -
組織特異性
All malignant melanoma cell lines tested and infrequently in glioma cell lines. -
配列類似性
Belongs to the MIA/OTOR family.
Contains 1 SH3 domain. -
翻訳後修飾
May possess two intramolecular disulfide bonds. -
細胞内局在
Secreted. - Information by UniProt
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別名
- Cartilage derived retinoic acid sensitive protein
- CD RAP
- CDRAP
see all -
参照データベース
- Entrez Gene: 8190 Human
- Omim: 601340 Human
- SwissProt: Q16674 Human
- Unigene: 646364 Human
画像
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Typical standard curve
Representative standard curve using ab171340
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab171340 は論文での使用が確認できていません。