Human Leptin ELISA Kit (ab179884)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 4.65 pg/ml
- Range: 15.63 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human Leptin ELISA Kit
Leptin キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Serum 8 5.1% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Serum 3 3.5% -
サンプルの種類
Cell culture supernatant, Serum, Plasma, Hep Plasma, EDTA Plasma, Cit plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
4.65 pg/ml -
検出範囲
15.63 pg/ml - 1000 pg/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Serum 91 90% - 93% Cell culture media 102 101% - 104% Hep Plasma 98 88% - 105% EDTA Plasma 82 80% - 87% Cit plasma 113 107% - 123% -
全工程の試験時間
1h 30m -
ステップ
One step assay -
種交差性
交差種: Human -
製品の概要
As of January 16, 2020, Human Leptin ELISA kit (ab179884) has been re-developed. We have identified new recombinant monoclonal antibodies to provide improved performance and consistency.
Human Leptin ELISA kit (ab179884) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Leptin protein in human serum, plasma and cell culture supernatants. It uses our proprietary SimpleStep ELISA® technology. Quantitate human Leptin with 4.65 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits. -
特記事項
Leptin is a secreted protein factor produced by adipocytes. It regulates energy balance and body fat deposits. Leptin deficiency in humans and mice can cause in obesity.Acute low levels of leptin triggers increased appetite and chronic leptin deficiency can block the onset of puberty.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 10 x 96 tests 10X Human Leptin Capture Antibody 1 x 600µl 1 x 600µl 10X Human Leptin Detector Antibody 1 x 600µl 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 1 x 20ml Antibody Diluent 4BI 1 x 6ml 1 x 6ml Human Leptin Lyophilized Recombinant Protein 2 vials 2 vials Plate Seals 1 unit 1 unit Sample Diluent NS (ab193972) 1 x 50ml 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit 1 unit Stop Solution 1 x 12ml 1 x 12ml TMB Development Solution 1 x 12ml 1 x 12ml -
研究分野
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機能
May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass. -
関連疾患
Defects in LEP may be a cause of obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat. -
配列類似性
Belongs to the leptin family. -
細胞内局在
Secreted. - Information by UniProt
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別名
- FLJ94114
- LEP
- LEP_HUMAN
see all -
参照データベース
- Entrez Gene: 3952 Human
- Omim: 164160 Human
- SwissProt: P41159 Human
- Unigene: 194236 Human
関連製品
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ELISA kits
画像
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Human Leptin redevelopment standard curve comparison
Standard Curve comparison between original human Leptin SimpleStep ELISA kit and redeveloped Simplestep ELISA kit. SimpleStep ELISA kit shows increased performance.
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SimpleStep ELISA Protocol DiagramSimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human Leptin standard curve in Sample Diluent NS.The Leptin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Leptin in human serum and plasma.The concentrations of Leptin were measured in duplicates, interpolated from the Leptin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 2.5%, plasma (citrate) 10%, plasma (edta) 10%, and plasma (heparin) 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Leptin concentration was determined to be 19,448 pg/mL in serum, 5,865 pg/mL in plasma (citrate), 9,377 pg/mL in plasma (edta), and 9,463 pg/mL in plasma (heparin).
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Interpolated concentrations of spike Leptin in cell culture media samples.The concentrations of Leptin were measured in duplicates, interpolated from the Leptin standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Serum from ten individual healthy human male donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Leptin concentration was determined to be 7,463 pg/mL with a range of 836 – 15,878 pg/mL.
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Sandwich ELISA - Human Leptin ELISA Kit (ab179884)To learn more about the advantages of SimpleStep ELISA® kits see here.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (8)
ab179884 は 8 報の論文で使用されています。
- Mir MM et al. Differential Association of Selected Adipocytokines, Adiponectin, Leptin, Resistin, Visfatin and Chemerin, with the Pathogenesis and Progression of Type 2 Diabetes Mellitus (T2DM) in the Asir Region of Saudi Arabia: A Case Control Study. J Pers Med 12:N/A (2022). PubMed: 35629157
- Wang D et al. Hepatokine Fetuin B expression is regulated by leptin-STAT3 signalling and associated with leptin in obesity. Sci Rep 12:12869 (2022). PubMed: 35896788
- Petrović B et al. Determination of salivary concentrations of leptin and adiponectin, ability to reduce ferric ions and total antioxidant capacity of saliva in patients with severe early childhood caries. Front Pediatr 10:969372 (2022). PubMed: 36120658
- Slaughter VL et al. Validation of an adipose-liver human-on-a-chip model of NAFLD for preclinical therapeutic efficacy evaluation. Sci Rep 11:13159 (2021). PubMed: 34162924
- Di Filippo L et al. Adiponectin to leptin ratio reflects inflammatory burden and survival in COVID-19. Diabetes Metab 47:101268 (2021). PubMed: 34333093
- Lorè NI et al. CXCL10 levels at hospital admission predict COVID-19 outcome: hierarchical assessment of 53 putative inflammatory biomarkers in an observational study. Mol Med 27:129 (2021). PubMed: 34663207
- Zhang T et al. Leptin Expression in Human Epicardial Adipose Tissue Is Associated with Local Coronary Atherosclerosis. Med Sci Monit 25:9913-9922 (2019). PubMed: 31872802
- Llobet L et al. OXPHOS xenobiotics alter adipogenic differentiation at concentrations found in human blood. Dis Model Mech N/A:N/A (2015). Functional Studies ; Human . PubMed: 26398948