Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
SimpleStep

Human IFN gamma ELISA Kit (ab174443)

Submit a review Submit a question References (14)
Other - Human IFN gamma ELISA Kit (ab174443)
  • Example IFNG standard curve for cell culture supernatant samples measurements.
  • Example of human Interferon-gamma standard curve in Sample Diluent NS (for supernatant samples)
  • Example IFNG standard curve for serum/plasma samples measurements.
  • Example of human Interferon-gamma standard curve in Sample Diluent NBP (for serum/plasma samples)
  • Comparison of secreted IFNG in unstimulated and PHA-stimulated Human PBMC.
  • Interpolated concentrations of secreted IFNG in unstimulated and PHA-stimulated Human PBMC.
  • Linearity of dilution.
  • Sandwich ELISA - Human IFN gamma ELISA Kit (ab174443)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 470 pg/ml
  • Range: 0.468 ng/ml - 30 ng/ml
  • Sample type: Cell culture supernatant, Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

製品の概要

  • 製品名

    Human IFN gamma ELISA Kit
    Interferon gamma キット 製品一覧

  • 検出方法

    Colorimetric

  • 再現性

    Intra-Assay（同時再現性）
    サンプル N 平均値 SD CV%
    Overall 5 1.1%
    Inter-Assay（日差再現性）
    サンプル N 平均値 SD CV%
    Overall 3 7.9%

  • サンプルの種類

    Cell culture supernatant, Serum, Plasma

  • アッセイタイプ

    Sandwich (quantitative)

  • 検出感度

    470 pg/ml

  • 検出範囲

    0.468 ng/ml - 30 ng/ml

  • 添加回収試験

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Serum 102 98% - 104%
    Cell culture media 86 77% - 93%
    Hep Plasma 244 197% - 298%
    EDTA Plasma 122 117% - 124%
    Cit plasma 107 96% - 115%

  • 全工程の試験時間

    1h 30m

  • ステップ

    One step assay

  • 種交差性

    交差種: Human

  • 製品の概要

    Human IFN gamma ELISA kit (ab174443) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IFN-gamma protein in human cell culture supernatant, plasma and serum samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate human IFN gamma with 470 pg/ml sensitivity.

    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

            -Single-wash protocol reduces assay time to 90 minutes or less
            -High sensitivity, specificity and reproducibility from superior antibodies
            -Fully validated in biological samples
            -96-wells plate breakable into 12 x 8 wells strips

    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits.


    ASSAY SPECIFICITY


    This kit recognizes both native and recombinant human Interferon-gamma protein in serum, plasma, and cell culture supernatant samples only.


    Urine, milk, and cell and tissue extract samples have not been tested with this kit.


    ASSAY INTERFERENCE


    This kit is incompatible with plasma (heparin) samples.

  • 特記事項

    IFN gamma (IFNG) is produced by lymphocytes activated by specific antigens or mitogens. IFN gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.

  • 試験プラットフォーム

    Microplate

製品の特性

画像

  • Other - Human IFN gamma ELISA Kit (ab174443)
    Other - Human IFN gamma ELISA Kit (ab174443)

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Example IFNG standard curve for cell culture supernatant samples measurements.
    Example IFNG standard curve for cell culture supernatant samples measurements.
    Background-subtracted data values (mean +/- SD) are graphed.
  • Example of human Interferon-gamma standard curve in Sample Diluent NS (for supernatant samples)
    Example of human Interferon-gamma standard curve in Sample Diluent NS (for supernatant samples)

    The Interferon-gamma standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • Example IFNG standard curve for serum/plasma samples measurements.
    Example IFNG standard curve for serum/plasma samples measurements.
    Background-subtracted data values (mean +/- SD) are graphed.
  • Example of human Interferon-gamma standard curve in Sample Diluent NBP (for serum/plasma samples)
    Example of human Interferon-gamma standard curve in Sample Diluent NBP (for serum/plasma samples)

    The Interferon-gamma standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • Comparison of secreted IFNG in unstimulated and PHA-stimulated Human PBMC.
    Comparison of secreted IFNG in unstimulated and PHA-stimulated Human PBMC.
    PBMC were grown in the absence or presence of phytohemagglutinin (PHA) for 2 days. IFNG concentrations were measured in 12X and 6X diluted cell culture supernatants of the unstimulated PBMC and the stimulated PBMC, and media. Raw data values (mean +/-SD, n=3) are graphed. The dotted line represents zero sample background.
  • Interpolated concentrations of secreted IFNG in unstimulated and PHA-stimulated Human PBMC.
    Interpolated concentrations of secreted IFNG in unstimulated and PHA-stimulated Human PBMC.
    The concentrations of IFNG were interpolated from data values shown in Figure 3 using IFNG standard curve and corrected for sample dilution. The mean IFNG concentration was determined to be 1.8 ng/mL in unstimulated PBMC supernatants and 177.2 ng/mL in stimulated PBMC supernatants.
  • Linearity of dilution.
    Linearity of dilution.

    Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.

    Native Interferon-gamma was measured in PHA-stimulated PBMC cell culture supernatant samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.
    Recombinant Interferon-gamma was spiked into human serum and plasma (citrate and EDTA) samples and diluted in a 2-fold dilution series in Sample Diluent NBP.

    Neat pooled serum and plasma (EDTA and Citrate) samples from healthy donors was measured in duplicate. All values were below the detectable range of the assay.

    Neat serum from ten individual healthy human female/male donors was measured in duplicate. All values were below the detectable range of the assay.

     

  • Sandwich ELISA - Human IFN gamma ELISA Kit (ab174443)
    Sandwich ELISA - Human IFN gamma ELISA Kit (ab174443)
    To learn more about the advantages of SimpleStep ELISA® kits see here.

参考文献 (14)

ab174443 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab174443 は 14 報の論文で使用されています。

  • Li S  et al. Hsa_circ_0048674 facilitates hepatocellular carcinoma progression and natural killer cell exhaustion depending on the regulation of miR-223-3p/PDL1. Histol Histopathol 37:1185-1199 (2022). PubMed: 35187630
  • Duan C  et al. RIP3 knockdown inhibits necroptosis of human intestinal epithelial cells via TLR4/MyD88/NF-?B signaling and ameliorates murine colitis. BMC Gastroenterol 22:137 (2022). PubMed: 35346043
  • Nayak D  et al. EMT alterations in the solute carrier landscape uncover SLC22A10/A15 imposed vulnerabilities in pancreatic cancer. iScience 25:104193 (2022). PubMed: 35479410
  • Huang Y  et al. Molecular mechanism of lncRNA SNHG12 in immune escape of non-small cell lung cancer through the HuR/PD-L1/USP8 axis. Cell Mol Biol Lett 27:43 (2022). PubMed: 35658874
  • Feng L  et al. Anti-PD-1 Therapy is Beneficial for the Survival of Patients with Oral Squamous Cell Carcinoma. Cancer Manag Res 14:2723-2731 (2022). PubMed: 36133741
View all Publications for this product

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

There are currently no Customer reviews or Questions for ab174443.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com