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リコンビナントSimpleStep

Human FLRG ELISA Kit (ab245715)

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Other - Human FLRG ELISA Kit (ab245715)
  • Example of human FLRG standard curve in Sample Diluent NS.
  • Example of human FLRG standard curve in Sample Diluent NS.
  • Interpolated concentrations of native FLRG in human serum and plasma samples.
  • Interpolated concentrations of native FLRG in human milk and urine samples.
  • Interpolated concentrations of native FLRG in JAR cell supernatant samples.
  • Serum from eight individual healthy human female donors was measured in duplicate.
  • Sandwich ELISA - Human FLRG ELISA Kit (ab245715)
  • Sandwich ELISA - Human FLRG ELISA Kit (ab245715)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 8.2 pg/ml
  • Range: 31.25 pg/ml - 2000 pg/ml
  • Sample type: Cell culture supernatant, EDTA Plasma, Hep Plasma, Milk, Serum, Urine
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

製品の概要

  • 製品名

    Human FLRG ELISA Kit
    FSTL3 キット 製品一覧

  • 検出方法

    Colorimetric

  • 再現性

    Intra-Assay（同時再現性）
    サンプル N 平均値 SD CV%
    serum 8 4.7%
    Inter-Assay（日差再現性）
    サンプル N 平均値 SD CV%
    serum 3 6.4%

  • サンプルの種類

    Cell culture supernatant, Milk, Urine, Serum, Hep Plasma, EDTA Plasma

  • アッセイタイプ

    Sandwich (quantitative)

  • 検出感度

    8.2 pg/ml

  • 検出範囲

    31.25 pg/ml - 2000 pg/ml

  • 添加回収試験

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 107 104% - 111%
    Milk 103 98% - 109%
    Urine 98 94% - 109%
    Serum 106 105% - 109%
    Hep Plasma 101 99% - 105%
    EDTA Plasma 104 102% - 106%

  • 全工程の試験時間

    1h 30m

  • ステップ

    One step assay

  • 種交差性

    交差種: Human
    非交差種: Cow

  • 製品の概要

    Human FLRG ELISA Kit (ab245715) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of FLRG protein in edta plasma, hep plasma, milk, serum, urine, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human FLRG with 8.2 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • 試験プラットフォーム

    Pre-coated microplate (12 x 8 well strips)

製品の特性

  • 保存方法

    Store at +4°C. Please refer to protocols.
  • 内容 1 x 96 tests
    10X Human FLRG Capture Antibody 1 x 600µl
    10X Human FLRG Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent 5BI 1 x 6ml
    Human FLRG Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml

  • 研究分野

  • 機能

    Isoform 1 or the secreted form is a binding and antagonizing protein for members of the TGF-beta family, such us activin, BMP2 and MSTN. Inhibits activin A-, activin B-, BMP2- and MSDT-induced cellular signaling; more effective on activin A than on activin B. Involved in bone formation; inhibits osteoclast differentiationc. Involved in hematopoiesis; involved in differentiation of hemopoietic progenitor cells, increases hematopoietic cell adhesion to fibronectin and seems to contribute to the adhesion of hematopoietic precursor cells to the bone marrow stroma. Isoform 2 or the nuclear form is probably involved in transcriptional regulation via interaction with MLLT10.

  • 組織特異性

    Expressed in a wide range of tissues.

  • 関連疾患

    Note=A chromosomal aberration involving FSTL3 is found in a case of B-cell chronic lymphocytic leukemia. Translocation t(11;19)(q13;p13) with CCDN1.

  • 配列類似性

    Contains 2 follistatin-like domains.
    Contains 2 Kazal-like domains.
    Contains 1 TB (TGF-beta binding) domain.

  • 細胞内局在

    Secreted and Nucleus. Although alternative initiation has been demonstrated and resulted in different localization, the major source of nuclear FSTL3 appears not to depend on translation initiation at Met-27 according to.
  • Information by UniProt

  • 別名

    • FLRG
    • follistatin-like 3
    • follistatin-like 3 glycoprotein
    • Follistatin-like protein 3
    • Follistatin-related gene protein
    • Follistatin-related protein 3
    • FSRP
    • Fstl3
    • FSTL3_HUMAN
    see all

  • 参照データベース

    画像

    • Other - Human FLRG ELISA Kit (ab245715)
      Other - Human FLRG ELISA Kit (ab245715)

      SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

       

    • Example of human FLRG standard curve in Sample Diluent NS.
      Example of human FLRG standard curve in Sample Diluent NS.
      Standard Curve comparison between human FLRG SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
    • Example of human FLRG standard curve in Sample Diluent NS.
      Example of human FLRG standard curve in Sample Diluent NS.
      The FLRG standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
    • Interpolated concentrations of native FLRG in human serum and plasma samples.
      Interpolated concentrations of native FLRG in human serum and plasma samples.
      The concentrations of FLRG were measured in duplicates, interpolated from the FLRG standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 15%, plasma (EDTA) 15%, and plasma (heparin) 15%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FLRG concentration was determined to be 8,804 pg/mL in serum, 8,996 pg/mL in plasma (EDTA) and 9,092 pg/mL in plasma (heparin).
    • Interpolated concentrations of native FLRG in human milk and urine samples.
      Interpolated concentrations of native FLRG in human milk and urine samples.
      The concentrations of FLRG were measured in duplicates, interpolated from the FLRG standard curves and corrected for sample dilution. Undiluted samples are as follows: milk 15% and urine 15%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FLRG concentration was determined to be 3,167 pg/mL in milk and 6,392 pg/mL in urine.
    • Interpolated concentrations of native FLRG in JAR cell supernatant samples.
      Interpolated concentrations of native FLRG in JAR cell supernatant samples.
      The concentrations of FLRG were measured in duplicates, interpolated from the FLRG standard curves and corrected for sample dilution. Undiluted samples are as follows: JAR cell supernatants 1%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FLRG concentration was determined to be 83,631 pg/mL in JAR cell supernatant.
    • Serum from eight individual healthy human female donors was measured in duplicate.
      Serum from eight individual healthy human female donors was measured in duplicate.

      Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FLRG concentration was determined to be 15,897 pg/mL with a range of 13,362 - 21,062 pg/mL.

    • Sandwich ELISA - Human FLRG ELISA Kit (ab245715)
      Sandwich ELISA - Human FLRG ELISA Kit (ab245715)
      To learn more about the advantages of recombinant antibodies see here.
    • Sandwich ELISA - Human FLRG ELISA Kit (ab245715)
      Sandwich ELISA - Human FLRG ELISA Kit (ab245715)
      To learn more about the advantages of SimpleStep ELISA® kits see here.

    参考文献 (0)

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    ab245715 は論文での使用が確認できていません。

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