Human Cripto ELISA Kit (ab245719)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 9.6 pg/ml
- Range: 26.56 pg/ml - 1700 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human Cripto ELISA Kit
Cripto1/CRIPTO キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% protein 8 1.7% -
サンプルの種類
Cell culture supernatant, Serum, EDTA Plasma, Cit plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
9.6 pg/ml -
検出範囲
26.56 pg/ml - 1700 pg/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 95 % - % Serum 86 % - % EDTA Plasma 86 % - % Cit plasma 86 % - % -
全工程の試験時間
1h 30m -
ステップ
One step assay -
種交差性
交差種: Human -
製品の概要
Human Cripto ELISA Kit (ab245719) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Cripto protein in cell culture supernatant, cit plasma, edta plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Cripto with 9.6 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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試験プラットフォーム
Pre-coated microplate (12 x 8 well strips)
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 10X Human Cripto Capture Antibody 1 x 600µl 10X Human Cripto Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human Cripto Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究分野
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機能
Could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm. -
組織特異性
Preferentially expressed in gastric and colorectal carcinomas than in their normal counterparts. -
配列類似性
Contains 1 EGF-like domain. -
細胞内局在
Cell membrane. - Information by UniProt
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別名
- CR
- CRGF
- cripto
see all -
参照データベース
- Entrez Gene: 6997 Human
- Omim: 187395 Human
- SwissProt: P13385 Human
- Unigene: 385870 Human
関連製品
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Alternative Versions
画像
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Other - Human Cripto ELISA Kit (ab245719)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human Cripto standard curve in Sample Diluent NS.The Cripto standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed. -
Interpolated concentrations of native Cripto in human NCCIT supernatant samples.The concentrations of Cripto were measured in duplicates, interpolated from the Cripto standard curves and corrected for sample dilution. Undiluted samples are as follows: NCCIT supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cripto concentration was determined to be 1.58 ng/mL in NCCIT supernatant. -
Interpolated concentrations of spiked Cripto in human serum and plasma samples.The concentrations of Cripto were measured in duplicates and interpolated from the Cripto standard curves. Undiluted samples are as follows: serum 12.5%, plasma (citrate) 12.5%, and plasma (EDTA) 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). -
Sandwich ELISA - Human Cripto ELISA Kit (ab245719)To learn more about the advantages of recombinant antibodies see here.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab245719 は論文での使用が確認できていません。