Human CD80 ELISA Kit (ab171342)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 62.5 pg/ml - 4000 pg/ml
- Sample type: Cell culture supernatant, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
-
製品名
Human CD80 ELISA Kit
CD80 キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Overall < 10% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Overall < 10% -
サンプルの種類
Cell culture supernatant, Serum -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 10 pg/ml -
検出範囲
62.5 pg/ml - 4000 pg/ml -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s Human CD80 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human CD80 in cell culture supernatants and serum.
A CD80 specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection monoclonal antibody from mouse, specific for CD80 is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human CD80 amount of sample captured in plate.
Produced using a non-baculovirus expression system.
-
試験プラットフォーム
Microplate
製品の特性
-
保存方法
Store at -20°C. Please refer to protocols. -
内容 ラベル 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml Antibody Diluent Buffer Green Cap 1 x 12ml Anti-Human CD80 Antibody Microplate (12 x 8 wells) 1 x 96 tests Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl Biotinylated anti-Human CD80 antibody 1 x 100µl Lyophilized recombinant Human CD80 standard 2 x 10ng Plate Seal 1 x 4 units Sample Diluent Buffer Green Cap 1 x 30ml TMB Color Developing Agent Amber Bottle 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml -
研究分野
-
機能
Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor. -
組織特異性
Expressed on activated B-cells, macrophages and dendritic cells. -
配列類似性
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
細胞内局在
Membrane. - Information by UniProt
-
別名
- Activation B7-1 antigen
- B lymphocyte activation antigen B7
- B7
see all -
参照データベース
- Entrez Gene: 941 Human
- Omim: 112203 Human
- SwissProt: P33681 Human
- Unigene: 838 Human
画像
-
Typical standard curve
Representative standard curve using ab171342
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab171342 は論文での使用が確認できていません。