Anti-Coxiella burnetii Phase 2 IgM ELISA Kit (Q-Fever) (ab178640)
Key features and details
- Sensitivity: 90 %
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Indirect
- Reacts with: Human
製品の概要
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製品名
Anti-Coxiella burnetii Phase 2 IgM ELISA Kit (Q-Fever) -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Pos serum 15 2.9% Pos serum 15 2% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Pos serum 6 7.7% Pos serum 6 2.4% -
サンプルの種類
Serum, Hep Plasma, Cit plasma -
アッセイタイプ
Indirect -
検出感度
> 90 % -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s anti-Coxiella burnetii (Q-Fever) Phase 2 IgM Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgM class antibodies against Coxiella burnetii in Human serum.
A 96-well plate has been precoated with Coxiella burnetii antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Coxiella burnetii antigens. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The intensity of yellow coloration is directly proportional to the amount of Coxiella burnetii IgG sample captured in plate.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 ラベル 1 x 96 tests 20X Washing Solution White cap 1 x 50ml Cover Foil 1 unit Coxiella burnetii Anti-IgM HRP Conjugate 1 x 20ml Coxiella burnetii IgM Cut-off Control 1 x 3ml Coxiella burnetii IgM Negative Control 1 x 2ml Coxiella burnetii IgM Positive Control 1 x 2ml Coxiella burnetii Phase 2 (IgM) Coated Microplate (12 x 8 wells) 1 unit IgM Sample Diluent 1 x 100ml Stop Solution red cap 1 x 15ml Strip holder 1 unit TMB Substrate Solution Yellow cap 1 x 15ml -
研究分野
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関連性
Coxiella burnetii is an obligate intracellular bacterial pathogen, and is the causative agent of Q fever. The genus Coxiella is morphologically similar to Rickettsia, but with a variety of genetic and physiological differences. C. burnetii is a small Gram-negative bacterium that is highly resistant to environmental stresses such as high temperature, osmotic pressure, and ultraviolet light. These characteristics are attributed to a small cell variant (SCV) form of the organism that is part of a biphasic developmental cycle, including a more metabolically and replicatively active large cell variant (LCV) form. It can survive standard disinfectants, and is resistant to many other environmental changes like those presented in the phagolysosome.
画像
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Serologic ELISA assay principleSpecific antigens are coated on the 96-well plate, controls or test samples are added to the well and incubated. The wells are washed to remove any unbound Human anti-antigen antibodies (Ig). A horseradish peroxidase (HRP) labelled anti-Human Ig conjugate is added to the wells. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The intensity of yellow coloration is directly proportional to the amount of Human anti-antigen Ig captured on the plate.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab178640 は論文での使用が確認できていません。