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CIC-C3d ELISA Kit (ab178665)

Submit a review Q&A (1)
Typical Standard Curve

    Key features and details

    • Sample type: Cit plasma, Hep Plasma, Serum
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Human

    製品の概要

    • 製品名

      CIC-C3d ELISA Kit

    • 検出方法

      Colorimetric

    • 再現性

      Intra-Assay（同時再現性）
      サンプル N 平均値 SD CV%
      Serum 16 6.1%
      Inter-Assay（日差再現性）
      サンプル N 平均値 SD CV%
      Serum 13.9%

    • サンプルの種類

      Serum, Hep Plasma, Cit plasma

    • アッセイタイプ

      Sandwich (quantitative)

    • ステップ

      Multiple steps standard assay

    • 種交差性

      交差種: Human

    • 製品の概要

      Abcam’s CIC-C3d in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of CIC-C3d in Human serum and plasma.

      A 96-well plate has been precoated with anti-C3d antibodies. Standards, controls and test samples are added to the wells and incubated. Immune complexes (CIC) in the samples and standards bind to the precoated antibody. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized CIC-C3d. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is directly proportional to the amount of CIC-C3d in the sample and the intensity is measured at 450 nm.

    • 試験プラットフォーム

      Microplate

    製品の特性

    • 保存方法

      Store at +4°C. Please refer to protocols.
    • 内容 1 x 96 tests
      10X Wash Solution 2 x 50ml
      Anti-CIC-C3d Coated Microplate (12 x 8 wells) 1 unit
      CIC-C3d Anti-IgG HRP Conjugate 1 x 0.5ml
      CIC-C3d Negative Control 1 x 1.5ml
      CIC-C3d Positive Control 1 x 1.5ml
      CIC-C3d Standard 0 - 0 µg Eq/mL 1 x 1.5ml
      CIC-C3d Standard 1 - 16 µg Eq/mL 1 x 1.5ml
      CIC-C3d Standard 2 - 64 µg Eq/mL 1 x 1.5ml
      Conjugate Buffer 1 x 20ml
      Cover Foil 1 unit
      Incubation Buffer 1 x 50ml
      Stop Solution 1 x 15ml
      Strip holder 1 unit
      TMB Substrate Solution 1 x 15ml

    • 研究分野

    画像

    • Typical Standard Curve
      Typical Standard Curve

      Representative standard curve using ab178665.

    参考文献 (0)

    ab178665 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab178665 は論文での使用が確認できていません。

    レビューと Q&A

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    レビューを送る 質問を送る

    Question

    The 3 standards included in the kit have ranges of concentrations. How does one use these 3 standards to generate a standard curve with the concentrations noted on page 13 if the exact concentration is not known?

    Read More
    Answer


    I apologize for the confusion with this protocol.

    Here are the concentrations:

    Standard 0 0 µg Eq/ml

    Standard 1 16 µg Eq/ml

    Standard 2 64 µg Eq/ml

    Read More

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