Chicken IgY ELISA Kit (ab157693)
Key features and details
- Sensitivity: 1.5305 ng/ml
- Range: 6.25 ng/ml - 400 ng/ml
- Sample type: Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Chicken
製品の概要
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製品名
Chicken IgY ELISA Kit
IgY キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Overall < 10% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Overall < 10% -
サンプルの種類
Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
1.5305 ng/ml -
検出範囲
6.25 ng/ml - 400 ng/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Serum > 85 % - % -
ステップ
Multiple steps standard assay -
種交差性
交差種: Chicken -
製品の概要
Abcam’s IgY Chicken ELISA kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for the quantitative measurement of IgY in chicken serum or plasma samples.
In this assay the IgY present in samples reacts with the anti-IgY antibodies which have been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, anti- IgY antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound IgY. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgY in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgY in the test sample. The quantity of IgY in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
Get results in 90 minutes with Chicken IgY ELISA Kit (ab189577) from our SimpleStep ELISA® range.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 100X HRP-conjugated anti-chicken IgY antibody 1 x 150µl 20X Wash Buffer Concentrate 1 x 50ml 5X Diluent Concentrate 1 x 50ml Chicken IgY Calibrator (Lyophilized) 1 vial Chicken IgY ELISA Microplate 1 unit Chromogen Substrate Solution 1 x 12ml Stop Solution 1 x 12ml -
研究分野
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関連性
Secreted immunoglobulins have two separable functions: one is to bind specifically to pathogenic molecules, the other is to recruit cells and molecules to destroy the opsonized pathogen. IgY has a tetrameric structure consisting of two identical light chains (Mr = 24 kDa) and two identical heavy chains (Mr = 73 kDa). -
別名
- FCR
- IGSF1
- immunoglobulin superfamily, member 1
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参照データベース
- Entrez Gene: 419114 Chicken
関連製品
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SimpleStep ELISA kits
画像
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Standard CurveRepresentative standard curve using ab157693 IgY Chicken ELISA Kit.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab157693 は 2 報の論文で使用されています。
- Lee KY et al. Development and characterization of a CRISPR/Cas9-mediated RAG1 knockout chicken model lacking mature B and T cells. Front Immunol 13:892476 (2022). PubMed: 36032098
- Wang WC et al. Supplementation of Bacillus subtilis-based probiotic reduces heat stress-related behaviors and inflammatory response in broiler chickens. J Anim Sci 96:1654-1666 (2018). PubMed: 29528406