Canine IgE ELISA Kit (ab157700)
Key features and details
- Sensitivity: 2.2897 ng/ml
- Range: 6.25 ng/ml - 400 ng/ml
- Sample type: Other biological fluids, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Dog
製品の概要
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製品名
Canine IgE ELISA Kit
IgE キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Overall < 10% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Overall < 10% -
サンプルの種類
Serum, Plasma, Other biological fluids -
アッセイタイプ
Sandwich (quantitative) -
検出感度
2.2897 ng/ml -
検出範囲
6.25 ng/ml - 400 ng/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Serum > 85 % - % -
ステップ
Multiple steps standard assay -
種交差性
交差種: Dog -
製品の概要
Abcam’s IgE Dog ELISA kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for the quantitative measurement of IgE in dog serum and plasma samples.
In this assay the IgE present in samples reacts with the anti-IgE antibodies which have been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, anti-IgE antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound IgE. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgE in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgE in the test sample. The quantity of IgE in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 100X HRP-conjugated anti-dog IgE antibody 1 x 150µl 20X Wash Buffer Concentrate 1 x 50ml 5X Diluent Concentrate 1 x 50ml Chromogen Substrate Solution 1 x 12ml Dog IgE Calibrator (lyophilized) 1 vial Dog IgE ELISA Microplate 1 unit Stop Solution 1 x 12ml -
研究分野
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関連性
IgE is the class of antibodies produced in the lungs, skin, and mucous membranes. It may protect against parasite invasion, but it is a major factor in allergic reactions. The antigen-specific IgE interacts with mast cells and eosinophils, triggers the release of histamine, leukotrienes and other substances that lead to the itching, sneezing and congestion of allergies - and the life threatening respiratory distress of asthma and anaphylactic shock. -
細胞内局在
Secreted -
別名
- Ig epsilon chain C region
- IgE
- IGHE
- immunoglobulin heavy constant epsilon
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参照データベース
画像
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Standard CurveRepresentative standard curve using ab157700 IgE Dog ELISA kit
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab157700 は 2 報の論文で使用されています。
- Pinto CFD et al. Hydrolyzed chicken liver used as single source of animal protein in diet and its effect on cytokines, immunoglobulins, and fecal microbiota profile of adult dogs. PLoS One 17:e0271932 (2022). PubMed: 35867776
- Koury J et al. Phosphodiesterase 4D, miR-203 and selected cytokines in the peripheral blood are associated with canine atopic dermatitis. PLoS One 14:e0218670 (2019). PubMed: 31226136