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リコンビナントSimpleStep

VILIP1 ELISA Kit (ab300324)

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Example of VILIP1 standard curve in Sample Diluent NS.
  • Interpolated concentrations of spiked VILIP1 in bovine cerebrospinal fluid (CSF) and human serum.
  • Interpolated concentrations of native VILIP1 in bovine and human CSF.
  • Sandwich ELISA - VILIP1 ELISA Kit (ab300324)
  • VILIP1 ELISA Kit (ab300324)
  • Sandwich ELISA - VILIP1 ELISA Kit (ab300324)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 18.144 pg/ml
  • Range: 39.063 pg/ml - 2500 pg/ml
  • Sample type: Cerebral Spinal Fluid, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Cow, Human

製品の概要

  • 製品名

    VILIP1 ELISA Kit
    VILIP1 キット 製品一覧

  • 検出方法

    Colorimetric

  • 再現性

    Intra-Assay（同時再現性）
    サンプル N 平均値 SD CV%
    Serum 8 6.7%
    Inter-Assay（日差再現性）
    サンプル N 平均値 SD CV%
    Serum 3 14.7%

  • サンプルの種類

    Serum, Cerebral Spinal Fluid

  • アッセイタイプ

    Sandwich (quantitative)

  • 検出感度

    18.144 pg/ml

  • 検出範囲

    39.063 pg/ml - 2500 pg/ml

  • 添加回収試験

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Serum 82 80% - 83%
    Cerebral Spinal Fluid 99 93% - 103%

  • 全工程の試験時間

    1h 30m

  • ステップ

    One step assay

  • 種交差性

    交差種: Cow, Human

  • 製品の概要

    VILIP1 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of VILIP1 protein in human serum and CSF samples. Quantitate  VILIP1 with 18.144 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


    -Single-wash protocol reduces assay time to 90 minutes or less
    -High sensitivity, specificity and reproducibility from superior antibodies
    -Fully validated in biological samples
    -96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • アプリケーション

    適用あり: Sandwich ELISAmore details

  • 試験プラットフォーム

    Pre-coated microplate (12 x 8 well strips)

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab300324の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Sandwich ELISA
Use at an assay dependent concentration.
特記事項
Sandwich ELISA
Use at an assay dependent concentration.

画像

  • Example of VILIP1 standard curve in Sample Diluent NS.
    Example of VILIP1 standard curve in Sample Diluent NS.

    Example of VILIP1 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of spiked VILIP1 in bovine cerebrospinal fluid (CSF) and human serum.
    Interpolated concentrations of spiked VILIP1 in bovine cerebrospinal fluid (CSF) and human serum.

    Recombinant VILIP1 was spiked into the following samples and diluted in a 2-fold dilution series in Sample Diluent NS. Undiluted samples are as follows: human serum 25% and bovine CSF 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

  • Interpolated concentrations of native VILIP1 in bovine and human CSF.
    Interpolated concentrations of native VILIP1 in bovine and human CSF.

    Interpolated concentrations of native VILIP1 were measured in duplicate in 50% bovine and human CSF. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

  • Sandwich ELISA - VILIP1 ELISA Kit (ab300324)
    Sandwich ELISA - VILIP1 ELISA Kit (ab300324)
    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
  • VILIP1 ELISA Kit (ab300324)
    VILIP1 ELISA Kit (ab300324)
  • Sandwich ELISA - VILIP1 ELISA Kit (ab300324)
    Sandwich ELISA - VILIP1 ELISA Kit (ab300324)
    To learn more about the advantages of SimpleStep ELISA® kits see here.

参考文献 (0)

ab300324 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab300324 は論文での使用が確認できていません。

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