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Testosterone ELISA Kit (ab108666)

Reviews (5)Q&A (10)References (58)
Biological fluids for ab108666
  • Typical Standard Curve

Key features and details

  • Sensitivity: 0.07 ng/ml
  • Range: 0.2 ng/ml - 16 ng/ml
  • Sample type: Cit plasma, Hep Plasma, Serum, Urine
  • Detection method: Colorimetric
  • Assay type: Competitive
  • Reacts with: Human

製品の概要

  • 製品名

    Testosterone ELISA Kit
    Testosterone キット 製品一覧

  • 検出方法

    Colorimetric

  • 再現性

    Intra-Assay（同時再現性）
    サンプル N 平均値 SD CV%
    Control Sera 48 <=5.8%
    Inter-Assay（日差再現性）
    サンプル N 平均値 SD CV%
    Control Sera 48 <=10.5%

  • サンプルの種類

    Urine, Serum, Hep Plasma, Cit plasma

  • アッセイタイプ

    Competitive

  • 検出感度

    = 0.07 ng/ml

  • 検出範囲

    0.2 ng/ml - 16 ng/ml

  • 添加回収試験

    = 105.5 %

  • ステップ

    Multiple steps standard assay

  • 種交差性

    交差種: Human

  • 製品の概要

    Testosterone ELISA kit is a competitive ELISA designed for the accurate quantitative measurement of Testosterone in serum and plasma.


    A 96-well plate has been precoated with anti-Testosterone antibodies. Samples and the Testosterone-HRP conjugate are added to the wells, where Testosterone in the sample competes with the added Testosterone-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Testosterone in the sample and the intensity is measured at 450 nm.

  • アプリケーション

    適用あり: Competitive ELISAmore details

  • 試験プラットフォーム

    Microplate

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab108666の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Competitive ELISA
Use at an assay dependent concentration.
特記事項
Competitive ELISA
Use at an assay dependent concentration.

画像

  • Biological fluids for ab108666
    Biological fluids for ab108666

    Testosterone measured in biological fluids showing quantity (ng) per mL of tested sample. Samples were diluted 1-2 fold.

  • Typical Standard Curve
    Typical Standard Curve

    Representative Standard Curve using ab108666

参考文献 (58)

ab108666 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab108666 は 58 報の論文で使用されています。

  • Stratos I  et al. Effects on Bone and Muscle upon Treadmill Interval Training in Hypogonadal Male Rats. Biomedicines 11:N/A (2023). PubMed: 37239040
  • Khairy EY & Salama OA Effect of exercise on the hypothalamic-pituitary-gonadal axis in a rat model of Alzheimer's disease. Sci Rep 13:14300 (2023). PubMed: 37653057
  • Kempińska-Podhorodecka A  et al. Effect of Low Testosterone Levels on the Expression of Proliferator-Activated Receptor Alpha in Female Patients with Primary Biliary Cholangitis. Cells 12:N/A (2023). PubMed: 37759496
  • Zhang Y  et al. Glucagon-like peptide-1 receptor agonists decrease hyperinsulinemia and hyperandrogenemia in dehydroepiandrosterone-induced polycystic ovary syndrome mice and are associated with mitigating inflammation and inducing browning of white adipose tissue†. Biol Reprod 108:945-959 (2023). PubMed: 36930063
  • Kelava I  et al. Androgens increase excitatory neurogenic potential in human brain organoids. Nature 602:112-116 (2022). PubMed: 35046577
View all Publications for this product

レビューと Q&A

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1-10 of 15 Abreviews or Q&A

Replacement for ab174569

 Good Excellent 5/5 (Ease of Use)
Abreviews
abreview image
Ordered as replacement for discontinued ab174569 for use on EDTA plasma (was informed ab108666 not yet validated for EDTA). Blood was collected in EDTA tubes (BD) and plasma separated by ficoll gradient was then span 2000g 10mins before aliquoting to freeze -80C. Samples were later thawed and assayed immediately, without dilution. ab108666 coped fine with samples >2ng/ml when measured by ab174569, although quantifying lower than ab174569, but samples <2ng/ml were all below LOD (set to 0.1ng/ml).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jul 11 2022

Dr Frances Orton

 Excellent Excellent 5/5 (Ease of Use)
Abreviews
abreview image
I used this product for common frog (Rana temporaria) urine. I first checked the levels of testosterone in the urine by measuring dilutions of 2 test samples against the highest and lowest concentrations of the standards. From this I was able to determine that the urine should be applied to the ELISA neat and did not require any dilution.
Levels of testosterone in the 88 samples that I tested were low, and in some cases below the lowest testosterone standard (see photograph).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Frances Orton

Verified customer

投稿 Sep 04 2020

Easy to use but maybe not suitable for mouse serum

 Below average Excellent 5/5 (Ease of Use)
Abreviews
We tried to use this kit to measure testosterone in mouse serum but were unable to measure anything in serum from normal WT mice. I suspect matrix effect interfered with measurements in neat or 1:2 diluted samples and more dilute samples (1 to 10) might have had levels below the detection limit. It is possible that further optimization or testing of other dilutions may help.
The standard worked really well and the product was easy to use. I would consider this kit if I ever have to measure testosterone in human samples but not for mouse.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Oct 23 2018

Works in guinea pig plasma

 Excellent Excellent 5/5 (Ease of Use)
Abreviews
abreview image
Bought this kit to try with guinea pig plasma. Kit was easy to use, fast and instructions were clear. From my results, it seems to have worked well in the guinea pig. Male samples all fell within the curve at expected concentrations.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 May 26 2017

Question

Does this kit ab108666 cross react with mouse testosterone as well?

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Answer

Testosterone is a biochemical and it should be same in all the species so theoretically this kit would work with testosterone from all the species. We however have tested it with human samples only. Please note the amount of testosterone is higher in urine as compared to serum and plasma samples.

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Question

I would like to know what solution to use to dilute samples since in the kit there is none.

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Answer

Samples can be diluted in PBS.

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Question

I have a couple of questions regarding this kit. First, is there a way to ascertain free testosterone from this kit as well, or is it necessary to perform a separate ELISA? Second, how many wells on the plate are used for controls?

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Answer

This assay will measure total testosterone, free and also protein-bound. To measure just free testosterone, we have another assay, https://www.abcam.com/human-testosterone-free-elisa-kit-ab178663.html.


We recommend running the two controls in duplicate, so those will require 4 wells. The five standards in duplicate will require 10 wells. One more well is needed for the substrate blank.

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Question

Which buffer can be used for diluting serum samples?

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Answer

I can confirm the lab used PBS to dilute serum samples when validating the kit.

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Question

I am using undiluted serum and plasma samples and observing high OD values. Do you have any suggestion why is this so?

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Answer

This is most likely due to matrix effect which affect measurement of analyte of interest in ELISA when undiluted serum or plasma samples are used. Matrix effect can arise from a number of matrix components such as phospholipids, carbohydrates, and endogenous metabolites (bilirubin), residual formulation components from intraperitoneal (ip), intravenous (iv), or oral dosing (po) experiments; for example, polyethylene glycol (PEG), solutol, polysorbate (Tween 80), etc, an interaction between the analyte of interest and the matrix, such as covalent binding to plasma proteins or the enzymatic degradation of a prodrug.  The easiest way to overcome this is by using diluted samples e.g. 1/2 or 1/10 dilution.

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Question

Does the assay detect both protein bound and free testosterone? Or is all testosterone considered bound to protein?

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Answer

The test measures both bound and free Testosterone.

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1-10 of 15 Abreviews or Q&A

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