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AB108666

Testosterone ELISA Kit

Testosterone ELISA Kit

4

(5 Reviews)

|

(69 Publications)

Testosterone ELISA kit is a competitive ELISA designed to quantify human testosterone in serum, plasma (citrate, heparin), and urine with a sensitivity of 0.07 ng/mL.

- Wide dynamic range – quantifies 0.2–16 ng/mL
- Designed to measure bound and free testosterone
- High recovery rate – achieves 105.5% general recovery
- Cited in over 55 publications

別名を表示する

17b hydroxy 4 androsten 3 one, 17鑴-hydroxyandrost-4-ene-3-one

3 Images
Competitive ELISA - Testosterone ELISA Kit (AB108666)
  • cELISA

Unknown

Competitive ELISA - Testosterone ELISA Kit (AB108666)

Representative Standard Curve using ab108666

Competitive ELISA - Testosterone ELISA Kit (AB108666)
  • cELISA

Lab

Competitive ELISA - Testosterone ELISA Kit (AB108666)

Testosterone measured in biological fluids showing quantity (ng) per mL of tested sample. Samples were diluted 1-2 fold.

ELISA - Testosterone ELISA Kit (AB108666)
  • ELISA

CiteAb

ELISA - Testosterone ELISA Kit (AB108666)

Testosterone ELISA Kit, ab108666. Publication image from Chen, J. et al., 2021, EMBO J, 33215738. Legend direct from paper.

Role of endothelial Gja1 in thyroid gland and testisRepresentative tile scan 3D images with staining forα‐SMA, Isolectin, Decorin and Emcn in adult (16 weeks old) thyroid gland from Gja1iδEC and littermate control mice. Bar graph (top right panel) with the quantification of vessel density in Gja1iδEC mutant and control thyroid gland. Bar graph (bottom right panel) with the level of thyroxine in the lysate of thyroid glands from Gja1iδEC and control mice determined by ELISA. Data represent mean ± s.d. (n = 5), P‐values and two‐tailed unpaired t‐tests.3D images with P‐Histone H3 and Emcn staining in Gja1iδEC and control thyroid gland. The scale bars are 50 µm.Bar graph with the quantification of P‐Histone H3+ EC numbers per 1mm2 sample areas in thyroid glands from Gja1iδEC and littermate control mice. Data represent mean ± s.d. (n = 5), P‐value and two‐tailed unpaired t‐test.Representative tile scan 3D images with staining of CD68, Caveolin‐1 and Endoglin in thyroid gland from Gja1iδEC and littermate control mice. Bar graph with the quantification of macrophage numbers in Gja1iδEC and control thyroid gland. Data represent mean ± s.d. (n = 5), P‐value and two‐tailed unpaired t‐test.Representative tile scan 3D images with staining of HSPG2, Isolectin and Endoglin in testis from Gja1iδEC and littermate control mice. Bar graph (top right panel) with the quantification of vessel density in Gja1iδEC mutant and control testis. Data represent mean ± s.d. (n = 5), P‐value and two‐tailed unpaired t‐test. Bar graph (bottom right panel) with the levels of testosterone in the lysate of testes from Gja1iδEC and control mice determined by ELISA. Data represent mean ± s.d. (n = 4), P‐value and two‐tailed unpaired t‐test.Data information : The white and dashed lines in panels A, D and E represent the outlines of organs. Nuclei : DAPI or TO‐PRO‐3 as indicated. Two‐tailed Student's t‐tests were performed for the statistical analysis. *P < 0.05; **P < 0.01. Scale bars are 200 µm for tile scan 3D images and 50 µm for higher magnification images.

Key facts

検出方法

Colorimetric

サンプルタイプ

Citrate plasma, Heparin Plasma, Serum, Urine

交差種

Human

アッセイタイプ

Competitive

検出感度

= 0.07 ng/mL

検出範囲

0.2 - 16 ng/mL

アッセイプラットフォーム

Microplate

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "cELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

製品の詳細

Testosterone ELISA Kit ab108666 is a competitive ELISA kit to measure human Testosterone (17β-Hydroxy-4-androstene-3-one) in serum, plasma (citrate, heparin), and urine with a sensitivity of 0.07 ng/mL.

How the assay works

Testosterone ELISA Kit ab108666 is a competitive ELISA. There are 2 types of competitive ELISAs:

  • antigen down: The sample antigen competes with a reference antigen for binding to a specific amount of labeled antibody. The reference antigen is pre-coated on a multi-well plate. The sample is added to the plate wells with a labeled antibody. Depending on the amount of antigen in the sample, more or fewer free antibodies will be available to bind the reference antigen. This means that the more of the antigen there is in the sample, the less reference antigen will be detected and the weaker the signal will be.
  • antibody down: the labeled antigen and the sample antigen (unlabeled) compete for binding to the primary antibody, which is immobilized on the plate. The lower the amount of antigen in the sample, the stronger the signal due to more labelled antigen in the well.

Assay specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility.

Testosterone ELISA Kit ab108666 protocol summary

  1. Add standards or samples to the wells, incubate 2 hrs
  2. Wash, then add 50 µl of Biotinylated Antibody per well. Incubate 1 hr
  3. Wash, then add 50 µl of SP Conjugate per well. Incubate 30 min
  4. Wash, then add 50 µl of Chromogen Substrate per well. Incubate 20 min
  5. Add 50 µl of Stop Solution per well. Read at 450 nm immediately

精度

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 48, "mean": null, "standardDeviation": null, "coefficientOfVariability": "<= 10.5" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 48, "mean": null, "standardDeviation": null, "coefficientOfVariability": "<= 5.8" } ]

製品内容

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出荷温度及び保存条件

出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
保管に関する情報
+4°C

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Testosterone also known as 17β-hydroxyandrost-4-en-3-one is a steroid hormone with a molecular mass of approximately 288.42 Da. It is primarily synthesized in the testes but also in smaller amounts in the ovaries and adrenal glands. Mechanically testosterone operates by binding to the androgen receptor a member of the nuclear receptor family which then modulates gene expression. Its synthesis is stimulated by luteinizing hormone (LH) which is released from the anterior pituitary gland.
Biological function summary

Testosterone plays an important role in the development of male secondary sexual characteristics such as increased muscle and bone mass and the growth of body hair. It's also part of the steroid hormone biosynthesis pathway influencing the production of estradiol through the aromatase enzyme. Testosterone contributes to the maintenance of reproductive tissues and is important for the modulation of mood and cognitive functions.

Pathways

Testosterone is integral to the HPG (hypothalamic-pituitary-gonadal) axis where it exerts feedback control by inhibiting the secretion of gonadotropin-releasing hormone (GnRH) and LH. Moreover testosterone is essential in the androgen receptor signaling pathway. This pathway involves other proteins like dihydrotestosterone (DHT) a potent androgen converted from testosterone via the 5α-reductase enzyme.

Low levels of testosterone commonly known as 'Low T' are linked to conditions like hypogonadism and certain metabolic syndromes. Testosterone levels can influence the risk of osteoporosis as sustained low levels reduce bone density. Additionally increased or decreased testosterone levels can pose risks to cardiovascular health with the target interacting with proteins such as sex hormone-binding globulin (SHBG) in these contexts.

製品プロトコール

ターゲットの情報

See full target information Testosterone

文献 (69)

Recent publications for all applications. Explore the full list and refine your search

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Differential Effects of Canonical Androgens and 11-Ketotestosterone on Reproductive Phenotypes and Folliculogenesis in Mouse Model of PCOS.

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Sahar Y Issa,S M Abdel Rahman,Yasmin M Gaber,Nada A H Soliman

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Thymoquinone-Loaded Chitosan Nanoparticles Combat Testicular Aging and Oxidative Stress Through SIRT1/FOXO3a Activation: An In Vivo and In Vitro Study.

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Enas A Kasem,Gehan Hamza,Nagi M El-Shafai,Nora F Ghanem,Shawky Mahmoud,Samy M Sayed,Mohammed Ali Alshehri,Laila A Al-Shuraym,Heba I Ghamry,Magdy E Mahfouz,Mustafa Shukry

Nutrients 16: PubMed39683564

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Effect of Elderberry ( L.) Extract Intake on Normalizing Testosterone Concentration in Testosterone Deficiency Syndrome Rat Model Through Regulation of 17β-HSD, 5α-Reductase, and CYP19A1 Expression.

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Jiyeon Kim,Jinho An,Youngcheon Song,Mincheol Jang,Hyunseok Kong,Sangbum Kim

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Aude Noiret,Fabienne Aujard,Jeremy Terrien

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Supplementing With Which Form of Creatine (Hydrochloride or Monohydrate) Alongside Resistance Training Can Have More Impacts on Anabolic/Catabolic Hormones, Strength and Body Composition?

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E Eghbali,H Arazi,K Suzuki

Journal of molecular neuroscience : MN 74:105 PubMed39496982

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Sex Differences in Blood Accumulation of Neurodegenerative-Related Proteins and Antioxidant Responses to Regular Physical Exercise.

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Elisa Chelucci,Giorgia Scarfò,Rebecca Piccarducci,Antonio Rizza,Jonathan Fusi,Francesco Epifani,Sara Carpi,Beatrice Polini,Laura Betti,Barbara Costa,Sabrina Taliani,Vito Cela,Paolo Artini,Simona Daniele,Claudia Martini,Ferdinando Franzoni

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Ink Mitigates Dehydroepiandrosterone-Induced Insulin Resistance in Mouse Model of Polycystic Ovarian Syndrome.

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Prathyusha Yamarthi,Rama Satyasri Kotipalli,Samatasai Patnaik,Kv Veena,Muralidharan Kathirvel,Rajkumar Vutukuri,Manjula Bhanoori
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