AB270552

SimpleStep ELISA® Custom ELISA Kit

SimpleStep
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(1 Publication)

SimpleStep ELISA^® Custom ELISA Kit allows you to develop your own SimpleStep ELISA^® using any antibody pair.

- No plate coating needed
- Possibility to measure different target on the same plate
- All components included, just add the antibody pair
- Streamline your ELISA® to a single-wash format if desired

4 Images

ELISA - SimpleStep ELISA® Custom ELISA Kit (AB270552)

ELISA

Supplier Data

ELISA - SimpleStep ELISA® Custom ELISA Kit (AB270552)

ELISA

Supplier Data

ELISA - SimpleStep ELISA® Custom ELISA Kit (AB270552)

Schematic representation of how the kit works

ELISA

Supplier Data

ELISA - SimpleStep ELISA® Custom ELISA Kit (AB270552)

SimpleStep ELISA® Custom ELISA Kit and conventional passive coating approach were compared. With ab270552, 10x less capture antibody can be used without affecting the assay performance.

ELISA

Supplier Data

ELISA - SimpleStep ELISA® Custom ELISA Kit (AB270552)

Other solution-phase binding ELISA formats typically use biotin to immobilize immunocomplexes. These can constrain the sample types that can be used with the assay. In contrast, ab270552 has been specifically designed to be used with most sample matrices without interference.

Key facts

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

SimpleStep ELISA® Custom ELISA Kit (ab270552) is the latest cutting-edge technology with a built-in system for antibody labeling. Built on Abcam’s patented CaptSure^TM technology this system enables the rapid and easy development of in-house ELISAs using any antibody pair, while eliminating the need to perform plate coating procedures.

It is powered by the combination of proprietary technologies, enabling you to reduce the amount of capture antibody required, measure different targets on the same plate with different assay antibody pairs, and streamline your ELISA to a single-wash format if desired.

The capture antibody of interest is quickly and easily conjugated with the CaptSure^TM Peptide supplied within the kit. The interaction between the antibody coated on the plate and the CaptSure^TM Peptide immobilizes the immune-complex.

The detection antibody is similarly conjugated with HRP using the Lightning-Link® HRP conjugation reagents, also supplied in the kit. Thus, with minimal hands-on time, you can set up both of your antibodies to ensure the best performance of your ELISA.

This kit has ready-to-go assay plates, pre-coated with antibody, which are specific for the CaptSure^TM Peptide.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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製品内容

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出荷温度及び保存条件

出荷温度

Ambient - Cannot Ship with Ice

短期保存温度

Multi

長期保存温度

Multi

保管に関する情報

Please refer to protocols

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製品プロトコール

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ターゲットの情報

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Cancer cell international 21:114 PubMed33596916

2021

Targeting SNHG3/miR-186-5p reverses the increased m6A level caused by platinum treatment through regulating METTL3 in esophageal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Mingxin Zhang,Minghua Bai,Li Wang,Ning Lu,Jia Wang,Rong Yan,Manli Cui,Honglin Yan,Lingmin Zhang

View all publications

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websiteProtocolBooklet

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

SimpleStep ELISA® Custom ELISA Kit