AB277286

SARS-CoV-2 (COVID-19) IgA ELISA Kit

Name: SARS-CoV-2 (COVID-19) IgA ELISA Kit (ab277286) | Abcam
Brand: Abcam Limited
SKU: AB277286
Price: 224000 JPY
Availability: InStock

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(1 Publication)

SARS-CoV-2 (COVID-19) IgA ELISA Kit is an indirect ELISA for the qualitative detection of IgA class antibodies against SARS-CoV-2 (COVID-19) in human plasma and serum samples.

- Colorimetric readout - 450 nm - Works on any standard plate reader
- Easy results interpretation

別名を表示する

Immunoglobulin alpha-2 heavy chain, Immunoglobulin alpha-2 heavy chain BUT

Key facts

検出方法

Colorimetric

サンプルタイプ

Citrate plasma, Heparin Plasma, Serum

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

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製品の詳細

SARS-CoV-2 (COVID-19) IgA ELISA Kit (ab277286) is intended for the qualitative determination of IgA class antibodies against SARS-CoV-2 in human serum or plasma (citrate, heparin) to support the diagnosis of COVID-19 disease and constitutes a supplement to direct pathogen detection. In addition, serology can be used to collect epidemiological information on the prevalence of SARS-CoV-2.

Microtiter plates are coated with specific antigens to bind corresponding antibodies of the sample. After washing the wells to remove all unbound sample material a horseradish peroxidase (HRP) labelled conjugate is added. This conjugate binds to the captured antibodies. In a second washing step unbound conjugate is removed. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is proportional to the amount of specific antibodies in the sample. Sulfuric acid is added to stop the reaction. This produces a yellow endpoint color. Absorbance at 450/620 nm is read using an ELISA Microtiter plate reader.

Antibody Isotypes and State of Infection:

Serology	Significance
IgM	Characteristic of the primary antibody response. High IgM titer: → suggests a current or very recent infection.
IgG	Follows IgM production. Characteristic of the secondary antibody response. May persist for several years. High IgG titer with low IgM titer: → may indicate past infection.
IgA	Produced in mucosal linings throughout the body (⇒ protective barrier). Usually produced early in the course of the infection.

Diagnostic Specificity:

The diagnostic specificity is defined as the probability of the assay of scoring negative in the absence of the specific analyte. SARS-CoV-2 infections emerged in December 2019 in Wuhan, China. The expected prevalence values for German and US blood donor panels from before December 2019 therefore amount to 0 %. The determined positive results correspond to a specificity of 98.26 % (95 %-confidence interval: 93.86 % - 99.79 %).

Sample panel	No. patients (n)	Positive	Equivocal	Negative	Specificity	95% CI
Blood donors Germany	66	0	0	66	100%
Blood donors US	50	2	1	47	95.92%
Total	116	2	1	113	98.26%	93.86%-99.79%

Diagnostic Sensitivity:

The diagnostic sensitivity is defined as the probability of the assay of scoring positive in the presence of the specific analyte. 42 samples from 25 patients tested positive for SARS-CoV-2 RNA by RT-PCR were tested.

Days post symptom onset	No. samples (n)	Positive	Equivocal	Negative	Sensitivity (Eqv excluded)
0-5	13	1	0	12	7.69%
6-8	10	4	1	5	44.44%
9-11	10	4	1	5	44.44%
greater than 12	9	8	1	0	100%

Interferences:

Three clinical samples exhibiting differing reactivities were tested for interference with each substance listed in the Table below: a positive, a negative, and an equivocal sample. All samples exhibited a change of signal less than 15 % when tested with each potential interferant.

Interferent	Conc. tested
Albumin	60 mg/mL
Bilirubin conjugated	0.4 mg/mL
Bilirubin unconugated	0.4 mg/mL
Cholesterol	4 mg/mL
Hemoglobin	10 mg/mL
Triglycerides	15 mg/mL

Cross Reactivity:

105 samples with antibody activities to potentially cross reacting parameters (including antibodies to several respiratory pathogens) were tested to evaluate the cross reactivity of the assay.

Samples +ve to antibodies to	No. of samples (n)	Positive	Equivocal	Negative
Adenovirus	10	0	1	9
Parainfluenzavirus	9	0	0	9
Candida albicans	8	0	0	8
Bordetella pertussis	9	0	0	9
Influenzavirus A	9	0	0	9
Influenzavirus B	10	0	1	9
Enterovirus	10	0	0	10
Respiratory syncytial virus	10	0	0	10
Chlamydia pneumoniae	9	0	0	9
Legionella pneumoniae	8	0	0	8
Mycoplasma pneumoniae	9	0	0	9
Haemophilus influenzae	3	0	0	3
Other Coronavirus	1	0	0	1

Cross reactions with antibodies to adenovirus and influenzavirus cannot be excluded. Cross reactivity with other human coronaviruses should be considered for result interpretation.

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精度

[ { "reproducibilityType": "Inter", "sample": "Sample 1", "replicates": 12, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.26" }, { "reproducibilityType": "Inter", "sample": "Sample 2", "replicates": 12, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.8" }, { "reproducibilityType": "Inter", "sample": "Sample 3", "replicates": 12, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.28" }, { "reproducibilityType": "Intra", "sample": "Sample 1", "replicates": 24, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.19" }, { "reproducibilityType": "Intra", "sample": "Sample 2", "replicates": 24, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.14" }, { "reproducibilityType": "Intra", "sample": "Sample 3", "replicates": 24, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.56" } ]

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製品内容

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The SARS-CoV-2 nucleocapsid protein also known as N protein plays a central role in the packaging and replication of the viral RNA genome. It functions mechanically by binding to the viral RNA to facilitate its replication and encapsidation. The nucleocapsid protein is expressed within the virus-infected cells particularly in the cytoplasm. It is a highly conserved structural protein of the virus and has a molecular weight of approximately 50 kDa. Additionally the nucleocapsid protein aids in RNA binding and is essential for the virus's structural stability.

Biological function summary

The nucleocapsid protein of SARS-CoV-2 is involved in maintaining the integrity of the viral genome. It forms a ribonucleoprotein complex with the viral RNA which is necessary for viral replication. The nucleocapsid protein is integral in the formation of the virus's structure and assists in the assembly and release of new virus particles. It interacts with other viral proteins contributing to the stability and functioning of the virus.

Pathways

The nucleocapsid protein is involved in the viral life cycle and replication pathways. The protein participates in the RNA replication and transcription complex working alongside proteins such as the RNA-dependent RNA polymerase and other non-structural proteins. These interactions within the pathways are essential for the efficient replication of the viral genome and production of new viral particles which are key steps in the virus's lifecycle.

The nucleocapsid protein is directly linked to COVID-19 the disease caused by the SARS-CoV-2 virus. It is a target for various diagnostic tests such as the COVID ELISA test and corona IgA test due to its immunogenic properties. The presence of nucleocapsid antibodies in patients is often measured through tests like the ELISA test which helps in diagnosing COVID-19. The nucleocapsid protein's involvement in COVID-19 progression connects it with other viral proteins highlighting its significance in disease mechanisms and potential therapeutic targeting.

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製品プロトコール

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ターゲットの情報

Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed : 20176268, PubMed : 22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed : 17576170, PubMed : 20176268). Ig alpha is the major immunoglobulin class in body secretions (PubMed : 2241915).

See full target information IGA2_HUMAN

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Nutrients 14: PubMed36014873

2022

Survival of Vaccine-Induced Human Milk SARS-CoV-2 IgG, IgA and SIgA Immunoglobulins across Simulated Human Infant Gastrointestinal Digestion.

Applications

Unspecified application

Species

Unspecified reactive species

Myrtani Pieri,Maria-Arsenia Maniori,Lucy Shahabian,Elie Kanaan,Irene Paphiti-Demetriou,Spyros Pipis,Kyriakos Felekkis,Vicky Nicolaidou,Christos Papaneophytou

View all publications

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websiteProtocolBooklet

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Abcam product promise

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SARS-CoV-2 (COVID-19) IgA ELISA Kit