Rat WISP1 ELISA Kit - Extracellular (ab290980)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 6.012 pg/ml
- Range: 15.625 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, EDTA Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
製品の概要
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製品名
Rat WISP1 ELISA Kit - Extracellular
WISP1 キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Serum 8 8.2% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Serum 4 9.1% -
サンプルの種類
Cell culture supernatant, Urine, Serum, EDTA Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
6.012 pg/ml -
検出範囲
15.625 pg/ml - 1000 pg/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 100 96% - 104% Urine 81 79% - 83% Serum 90 85% - 95% EDTA Plasma 94 86% - 106% Cit plasma 81 80% - 82% -
全工程の試験時間
1h 30m -
ステップ
One step assay -
種交差性
交差種: Rat -
製品の概要
Rat WISP1 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of WISP1 protein in Rat serum, plasma, cell culture supernatant and urine. Quantitate Rat WISP1 with 6.012 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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アプリケーション
適用あり: Sandwich ELISAmore details -
試験プラットフォーム
Pre-coated microplate (12 x 8 well strips)
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 10X Rat WISP1 Capture Antibody 1 x 600µl 10X Rat WISP1 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BR 1 x 6ml Plate Seal 1 unit Rat WISP1 Lyophilized Recombinant Protein 2 vials Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究分野
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関連性
WISP1 is a downstream regulator in the Wnt/Frizzled-signaling pathway and is associated with cell survival. WISP1 attentuates p53-mediated apoptosis in response to DNA damage through activation of AKT kinase and up-regulates the anti-apoptotic Bcl-X(L) protein. WISP1 is expressed in heart, kidney, lung, pancreas, placenta, ovary, small intestine and spleen. Overexpression is associated with several cancers including breast cancer and colon tumors. -
細胞内局在
Secreted -
別名
- CCN 4
- CCN family member 4
- CCN4
see all -
参照データベース
- Entrez Gene: 65154 Rat
- SwissProt: Q99PP0 Rat
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab290980の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
画像
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Sandwich ELISA - Rat WISP1 ELISA Kit (ab290980)Example of rat WISP1 standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.
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Sandwich ELISA - Rat WISP1 ELISA Kit (ab290980)Interpolated concentration of native WISP1 was measured in duplicate at different sample concentrations. Undiluted samples are serum 50%, plasma (EDTA) 100%, and plasma (citrate) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS. -
Sandwich ELISA - Rat WISP1 ELISA Kit (ab290980)Interpolated concentration of native WISP1 was measured in duplicate at different sample concentrations. Undiluted samples are 25% L6 cell culture supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS. -
Sandwich ELISA - Rat WISP1 ELISA Kit (ab290980)Standard curve comparison between Rat WISP1 SimpleStep ELISA kit (ab290980) and traditional ELISA kit from leading competitor. The SimpleStep ELISA kit shows comparable sensitivity. -
Rat WISP1 ELISA Kit (ab290980) -
Rat WISP1 ELISA Kit (ab290980)
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab290980 は論文での使用が確認できていません。