AB255716

Rat ST2 ELISA Kit (IL1RL1)

Recombinant
SimpleStep
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(3 Publications)

Rat ST2 ELISA Kit (IL1RL1) is a single-wash 90-min Simplestep used to quantify Rat ST2 (IL1RL1) with a sensitivity of 5.36 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

別名を表示する

Fit1, Il1rl1, Interleukin-1 receptor-like 1, Fit-1, Fos-responsive gene 1 protein

2 Images

Sandwich ELISA - Rat ST2 ELISA Kit (IL1RL1) (AB255716)

sELISA

Supplier Data

Sandwich ELISA - Rat ST2 ELISA Kit (IL1RL1) (AB255716)

Example of rat ST2 standard curve in Sample Diluent NS.

The ST2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Rat ST2 ELISA Kit (IL1RL1) (AB255716)

Interpolated concentrations of native ST2 in rat serum and plasma samples.

The concentrations of ST2 were measured in duplicates, interpolated from the ST2 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 100%, plasma (citrate) 100%, plasma (EDTA) 50% and plasma (heparin) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ST2 concentration was determined to be 656.39 pg/mL in serum, 767.97 pg/mL in plasma (citrate), 534.46 pg/mL in plasma (EDTA), and 531.74 pg/mL in plasma (heparin).

Key facts

検出方法

Colorimetric

サンプルタイプ

Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

交差種

Rat

アッセイタイプ

Sandwich

Quantitative

検出感度

= 5.36 pg/mL

検出範囲

70.31 - 4500 pg/mL

アッセイ時間

1h 30m

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Rat ST2 ELISA Kit (IL1RL1) (ab255716) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of ST2 (IL1RL1) protein in cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat ST2 (IL1RL1) with 5.36 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

ST2, also known as or Interleukin 1 receptor-like 1 (IL-1RL1), is a protein encoded by the IL1RL1 gene, a member of the interleukin 1 receptor family, specifically the receptor for interleukin-33. IL-33 binding to ST2 induces MYD88, IRAK1, IRAK4, and TRAF6 recruitment and subsequent phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Studies of the homologous gene in mouse reported protein induction by pro-inflammatory stimuli with involvement in the function of helper T cells. ST2 is necessary for endotoxin tolerance by inhibiting TLR responses inducing the Th2 response. Rat ST2 shares 80.1%, 63.3%, 52.9%, and 61.6% sequence homology with mouse, human, bovine, and monkey, respectively.

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精度

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3" } ]

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回収率

[ { "sample": "Serum", "range": "81 - 83 %", "average": "= 82" }, { "sample": "EDTA Plasma", "range": "81 - 102 %", "average": "= 90" }, { "sample": "Heparin Plasma", "range": "83 - 94 %", "average": "= 88" }, { "sample": "Citrate plasma", "range": "81 - 95 %", "average": "= 90" } ]

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製品内容

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

ST2 also known as IL-1RL1 or IL-1RL1 chimera protein is a member of the IL-1 receptor family. It has a molecular mass of approximately 55 kDa. This protein exists in different forms including a membrane-bound form and a soluble variant. Researchers typically find ST2 expressed in various tissues including the lungs gut and cardiovascular system. The expression level can vary significantly depending on the physiological or pathological state.

Biological function summary

ST2 interacts with interleukin-33 (IL-33) and functions as its receptor. The binding of IL-33 to ST2 activates immune responses particularly influencing T-helper 2 (Th2) cells. ST2 forms part of a receptor complex including IL-1 receptor accessory protein IL-1RAcP necessary for signal transduction. This interaction leads to the activation of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) and mitogen-activated protein kinase (MAPK) signaling pathways facilitating inflammatory responses.

Pathways

IL-33 signals through the ST2 receptor participating actively in the immune signaling pathway. This pathway plays a significant role in modulating inflammatory and immune responses. ST2 association with NF-kB broadly affects inflammatory signaling while its involvement with the MAPK pathway aids in cellular responses to cytokines and stress. The close relationship with the IL-33/ST2 axis highlights its importance in maintaining immune homeostasis and reacting to environmental stressors.

ST2 has significant associations with asthma and cardiovascular diseases. In asthma the IL-33/ST2 axis critically contributes to airway inflammation and hyperresponsiveness. Similarly in the context of cardiovascular diseases elevated levels of soluble ST2 are markers of heart failure with the ST2/IL-33 axis contributing to cardiac remodeling. ST2's connection with inflammatory processes implies it also interacts with other cytokines such as interleukin-6 (IL-6) exacerbating the disease states through shared pathways.

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製品プロトコール

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ターゲットの情報

Receptor for interleukin-33 (IL-33) which plays crucial roles in innate and adaptive immunity, contributing to tissue homeostasis and responses to environmental stresses together with coreceptor IL1RAP. Its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Possibly involved in helper T-cell function (By similarity). Upon tissue injury, induces UCP2-dependent mitochondrial rewiring that attenuates the generation of reactive oxygen species and preserves the integrity of Krebs cycle required for persistent production of itaconate and subsequent GATA3-dependent differentiation of inflammation-resolving alternatively activated macrophages.. Isoform B. Inhibits IL-33 signaling.

See full target information Il1rl1

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

American journal of physiology. Heart and circulatory physiology 326:H1006-H1016 PubMed38363211

2024

IL-33 supplementation improves uterine artery resistance and maternal hypertension in response to placental ischemia.

Applications

Unspecified application

Species

Unspecified reactive species

Xi Wang,Corbin Shields,Geilda Tardo,Greg Peacock,Emily Hester,Marissa Anderson,Jan M Williams,Denise C Cornelius

Frontiers in cardiovascular medicine 10:1181473 PubMed37383701

2023

Optimal treatment for post-MI heart failure in rats: dapagliflozin first, adding sacubitril-valsartan 2 weeks later.

Applications

Unspecified application

Species

Unspecified reactive species

Wenqi Tao,Xiaoyu Yang,Qing Zhang,Shuli Bi,Zhuhua Yao

Frontiers in pharmacology 14:1074837 PubMed37089931

2023

Linggui Zhugan Decoction activates the SIRT1-AMPK-PGC1α signaling pathway to improve mitochondrial and oxidative damage in rats with chronic heart failure caused by myocardial infarction.

Applications

Unspecified application

Species

Unspecified reactive species

Siyi Yu,Hang Qian,Dawei Tian,Mingming Yang,Dongfeng Li,Hao Xu,Jishun Chen,Jingning Yang,Xincai Hao,Zhixin Liu,Jixin Zhong,Handong Yang,Xinlong Chen,Xinwen Min,Jun Chen

View all publications

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websiteProtocolBooklet

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Rat ST2 ELISA Kit (IL1RL1)