AB325077

Rat Parathyroid Hormone (PTH) ELISA Kit

SimpleStep
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Rat Parathyroid Hormone (PTH) ELISA Kit is a single-wash 90-min Simplestep used to quantify Rat Parathyroid Hormone (PTH) with a sensitivity of 16pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

-Colormetric sandwich ELISA - 450 nm readout- works on any standard plate reader.

-Different formats for different needs: 10 x 96 plates for bulk orders and 384-well for higher throughput.

別名を表示する

Parathyroid hormone, PTH, Parathyrin

4 Images

Sandwich ELISA - Rat Parathyroid Hormone (PTH) ELISA Kit (AB325077)

sELISA

Lab

Sandwich ELISA - Rat Parathyroid Hormone (PTH) ELISA Kit (AB325077)

Example of rat Parathyroid Hormone standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Lab

Sandwich ELISA - Rat Parathyroid Hormone (PTH) ELISA Kit (AB325077)

Interpolated concentration of native Parathyroid Hormone was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 10% mouse thymus supernatant. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Lab

Sandwich ELISA - Rat Parathyroid Hormone (PTH) ELISA Kit (AB325077)

Interpolated concentration of native Parathyroid Hormone was measured in duplicate at different sample concentrations. Undiluted samples are 10% rat thymus tissue supernatant and 10% mouse thymus tissue supernatant. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Lab

Sandwich ELISA - Rat Parathyroid Hormone (PTH) ELISA Kit (AB325077)

Example of rat Parathyroid Hormone standard curve in 96-well vs. 384-well plate in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

検出方法

Colorimetric

サンプルタイプ

Serum, Citrate plasma, EDTA Plasma, Supernatant, Cell culture media

交差種

Rat

アッセイタイプ

Sandwich

Quantitative

検出感度

= 16 pg/mL

検出範囲

18.75 - 1200 pg/mL

アッセイ時間

1h 30m

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Rat Parathyroid Hormone (PTH) ELISA Kit (ab325077) is a rapid single-wash sandwich 90-min ELISA to measure Rat Parathyroid Hormone (PTH) in Serum, Plasma – Citrate, Plasma – EDTA, Supernatant, Cell Culture Media. This SimpleStep sensitivity is 16 pg/ml.

How the assay works

Rat Parathyroid Hormone (PTH) ELISA Kit (ab325077) SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Rat Parathyroid Hormone (PTH) (ab325077) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Save your precious samples

Our Rat Parathyroid Hormone (PTH) ELISA Kit only requires 12.5µL sample.

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精度

[ { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 6.9" }, { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 8.4" } ]

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回収率

[ { "sample": "Serum", "range": "86 - 98 %", "average": "= 92" }, { "sample": "Citrate plasma", "range": "82 - 86 %", "average": "= 84" }, { "sample": "EDTA Plasma", "range": "86 - 96 %", "average": "= 91" }, { "sample": "Cell culture supernatant", "range": "95 - 98 %", "average": "= 96" }, { "sample": "Cell culture media", "range": "84 - 88 %", "average": "= 86" } ]

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製品内容

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab325077-10x96Tests", "assetComponentDetails": [ { "size":"10 x 1 Unit", "name":"Plate Seal", "number":"AB325077-CMP11" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB325077-CMP09" }, { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB325077-CMP08" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB325077-CMP07" }, { "size":"2 x 250 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB325077-CMP06" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB325077-CMP05" }, { "size":"10 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB325077-CMP04" }, { "size":"20 x 1 Vial", "name":"Rat Parathyroid Hormone (PTH) Lyophilized Recombinant Protein", "number":"AB325077-CMP03" }, { "size":"10 x 600 µL", "name":"10X Rat Parathyroid Hormone (PTH) Detector Antibody", "number":"AB325077-CMP02" }, { "size":"10 x 600 µL", "name":"10X Rat Parathyroid Hormone (PTH) Capture Antibody", "number":"AB325077-CMP01" } ] }, "1x384Tests": { "sellingSize": "1 x 384 Tests", "publicAssetCode":"ab325077-1x384Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB325077-CMP11" }, { "size":"2 x 12 mL", "name":"Stop Solution", "number":"AB325077-CMP08" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB325077-CMP05" }, { "size":"1 x 1 Unit", "name":"384 well CaptSure™ microplates", "number":"AB325077-CMP10" }, { "size":"2 x 12 mL", "name":"TMB Development Solution", "number":"AB325077-CMP07" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB325077-CMP06" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB325077-CMP04" }, { "size":"2 x 1 Vial", "name":"Rat Parathyroid Hormone (PTH) Lyophilized Recombinant Protein", "number":"AB325077-CMP03" }, { "size":"1 x 600 µL", "name":"10X Rat Parathyroid Hormone (PTH) Detector Antibody", "number":"AB325077-CMP02" }, { "size":"1 x 600 µL", "name":"10X Rat Parathyroid Hormone (PTH) Capture Antibody", "number":"AB325077-CMP01" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab325077-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB325077-CMP11", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB325077-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB325077-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB325077-CMP07", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB325077-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB325077-CMP05", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB325077-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Rat Parathyroid Hormone (PTH) Lyophilized Recombinant Protein", "number":"AB325077-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Rat Parathyroid Hormone (PTH) Detector Antibody", "number":"AB325077-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Rat Parathyroid Hormone (PTH) Capture Antibody", "number":"AB325077-CMP01", "productcode":"" } ] } } }

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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製品プロトコール

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ターゲットの情報

Parathyroid hormone elevates calcium level by dissolving the salts in bone and preventing their renal excretion. Acts by binding to its receptor, PTH1R, activating G protein-coupled receptor signaling. Stimulates [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblastic cells.

See full target information Pth

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websiteProtocolBooklet

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Rat Parathyroid Hormone (PTH) ELISA Kit