AB100778

Rat MCP1 ELISA Kit (CCL2)

Name: Rat MCP1 ELISA Kit (CCL2) (ab100778) | Abcam
Brand: Abcam Limited
SKU: AB100778
Price: 109000 JPY
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(13 Publications)

Rat MCP1 ELISA Kit (CCL2) is a Sandwich ELISA for the measurement of Rat MCP1 (CCL2) in Rat in Cell/Tissue Extracts samples.

別名を表示する

Je, Mcp1, Scya2, Ccl2, C-C motif chemokine 2, Immediate-early serum-responsive protein JE, Monocyte chemoattractant protein 1, Monocyte chemotactic protein 1, Small-inducible cytokine A2, MCP-1

1 Images

Sandwich ELISA - Rat MCP1 ELISA Kit (CCL2) (AB100778)

sELISA

Unknown

Sandwich ELISA - Rat MCP1 ELISA Kit (CCL2) (AB100778)

Representative standard curve using ab100778

Key facts

検出方法

Colorimetric

サンプルタイプ

Cell culture extracts, Tissue Extracts

交差種

Rat

アッセイタイプ

Sandwich

検出感度

< 15 pg/mL

検出範囲

24.67 - 18000 pg/mL

アッセイプラットフォーム

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Abcam's MCP1 Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat MCP1 in cell lysates and tissue lysates.

This assay employs an antibody specific for rat MCP1 coated on a 96-well plate. Standards and samples are pipette into the wells and MCP1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat MCP1 antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MCP1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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回収率

[ { "sample": "Cell Lysate", "range": "84 - 105 %", "average": "= 93.73" }, { "sample": "Tissue Lysate", "range": "83 - 103 %", "average": "= 92.75" } ]

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製品内容

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100778-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB100778-CMP05", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100778-CMP02", "productcode":"" }, { "size":"1 x 5 mL", "name":"2X Cell Lysis Buffer", "number":"AB100778-CMP09", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100778-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant rat MCP1 Standard (lyophilized)", "number":"AB100778-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"MCP1 Microplate (12 x 8 wells)", "number":"AB100778-CMP03", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Sample Diluent Buffer", "number":"AB100778-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100778-CMP07", "productcode":"" }, { "size":"1 x 200 µL", "name":"100X HRP-Streptavidin Concentrate", "number":"AB100778-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Rat MCP1", "number":"AB100778-CMP10", "productcode":"" } ] } } }

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

-20°C

長期保存温度

-20°C

保管に関する情報

-20°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

MCP-1 also known as CCL2 is a chemokine involved in the recruitment of monocytes to sites of injury and inflammation. This protein consists of 76 amino acids and has a molecular weight of about 11 kDa. MCP-1 is expressed in a wide range of cell types including endothelial cells fibroblasts and monocytes. It acts by binding to the chemokine receptor CCR2 stimulating intracellular signaling pathways that mediate cell migration.

Biological function summary

The role of MCP-1 is significant in guiding immune cells to areas requiring immune response. It does not form part of a complex but operates as a singular entity to affect immune signaling. MCP-1 influences the behavior of monocytes and macrophages by inducing chemotaxis and activating their effector functions. Through this mechanism it aids in the inflammatory response and tissue repair.

Pathways

MCP-1 participates in the inflammatory pathways by cooperating with key proteins like CCR2. It facilitates the JAK-STAT signaling pathway which is vital for transmitting extracellular information into cellular responses. The interaction of MCP-1 with CCR2 influences the activation of downstream proteins like STAT3 enabling various immune responses. MCP-1 thereby plays a central role in modulating inflammation-related pathways.

MCP-1 has a connection to inflammatory bowel disease and atherosclerosis. In such conditions elevated levels of MCP-1 can exacerbate inflammatory responses by recruiting monocytes to dysfunctional sites worsening tissue damage. Through its interaction with CCR2 MCP-1 becomes a pivotal factor in chronic inflammation. Targeting this MCP-1/CCR2 axis can provide therapeutic opportunities for modulating disease progression in these inflammatory diseases.

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製品プロトコール

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ターゲットの情報

Acts as a ligand for C-C chemokine receptor CCR2 (By similarity). Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions (By similarity). Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils (By similarity). Plays an important role in mediating peripheral nerve injury-induced neuropathic pain (By similarity). Increases NMDA-mediated synaptic transmission in both dopamine D1 and D2 receptor-containing neurons, which may be caused by MAPK/ERK-dependent phosphorylation of GRIN2B/NMDAR2B (By similarity).

See full target information Ccl2

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文献 (13)

Recent publications for all applications. Explore the full list and refine your search

Theranostics 14:608-621 PubMed38169629

2024

Inactivation of the NLRP3 inflammasome mediates exosome-based prevention of atrial fibrillation.

Applications

Unspecified application

Species

Unspecified reactive species

Sandrine Parent,Ramana Vaka,Jennifer St Amant,Saad Kahn,Sophie Van Remortel,Christina Bi,David Courtman,Duncan John Stewart,Darryl Raymond Davis

JCI insight 8: PubMed37384420

2023

Prevention of atrial fibrillation after open-chest surgery with extracellular vesicle therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Sandrine Parent,Ramana Vaka,Yousef Risha,Clarissa Ngo,Pushpinder Kanda,Stanley Nattel,Saad Khan,David Courtman,Duncan J Stewart,Darryl R Davis

Frontiers in cardiovascular medicine 9:981333 PubMed36818914

2023

Angiotensin receptor-neprilysin inhibitor improves coronary collateral perfusion.

Applications

Unspecified application

Species

Unspecified reactive species

Kangbo Li,Victoria Kratzmann,Mengjun Dai,Nora Gatzke,Petra Rocic,Peter Bramlage,Olaf Grisk,Lubomir T Lubomirov,Meike Hoffmeister,Martin A Lauxmann,Oliver Ritter,Eva Buschmann,Michael Bader,Anja Bondke Persson,Ivo Buschmann,Philipp Hillmeister

Fundamental & clinical pharmacology 37:60-74 PubMed36117326

2022

Activation of the Mas receptors by AVE0991 and MrgD receptor using alamandine to limit the deleterious effects of Ang II-induced hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Lokman Hekim Tanrıverdi,Onural Özhan,Ahmet Ulu,Azibe Yıldız,Burhan Ateş,Nigar Vardı,Hacı Ahmet Acet,Hakan Parlakpinar

Advances in clinical and experimental medicine : official organ Wroclaw Medical University 30:967-980 PubMed34418332

2021

Extracellular Nampt (eNampt/visfatin/PBEF) directly and indirectly stimulates ACTH and CCL2 protein secretion from isolated rat corticotropes.

Applications

Unspecified application

Species

Unspecified reactive species

Piotr Celichowski,Karol Jopek,Marta Szyszka,Paulina Milecka,Marianna Tyczewska,Svetlana Sakhanova,Witold Szaflarski,Ludwik Kazimierz Malendowicz,Marcin Ruciński

Molecular medicine reports 21:1623-1632 PubMed32016482

2020

Monocyte chemoattractant protein‑1 promotes the proliferation, migration and differentiation potential of fibroblast‑like synoviocytes via the PI3K/P38 cellular signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiang Tong,Huangjian Zeng,Pengchen Gu,Kai Wang,Han Zhang,Xiangjin Lin

Anesthesiology 132:343-356 PubMed31939850

2020

Oral Dimethyl Fumarate Reduces Peripheral Neuropathic Pain in Rodents via NFE2L2 Antioxidant Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Jiahe Li,Jiacheng Ma,Michael J Lacagnina,Sabina Lorca,Max A Odem,Edgar T Walters,Annemieke Kavelaars,Peter M Grace

Journal of the American Heart Association 9:e014977 PubMed31880979

2019

Renal Dopamine Oxidation and Inflammation in High Salt Fed Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Anees A Banday,Mustafa F Lokhandwala

Iranian journal of basic medical sciences 22:884-892 PubMed31579444

2019

Pioglitazone abrogates testicular damage induced by testicular torsion/detorsion in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Nevertyty Mohamed Mahmoud,Soad Lotfy Kabil

Frontiers in pharmacology 9:331 PubMed29681857

2018

A Synthesized Glucocorticoid- Induced Leucine Zipper Peptide Inhibits Retinal Müller Cell Gliosis.

Applications

Unspecified application

Species

Unspecified reactive species

Ruiping Gu,Xinyi Ding,Wenyi Tang,Boya Lei,Chen Jiang,Gezhi Xu

View all publications

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websiteProtocolBooklet

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Abcam product promise

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Rat MCP1 ELISA Kit (CCL2)