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AB46073

Rat IL-4 ELISA Kit

Rat IL-4 ELISA Kit

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Rat IL-4 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Rat IL-4 in Rat in Cell Culture Media, Biofluids samples.

別名を表示する

Il-4, Il4, Interleukin-4, IL-4, B-cell IGG differentiation factor, B-cell growth factor 1, B-cell stimulatory factor 1, Lymphocyte stimulatory factor 1, BSF-1

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Sandwich ELISA - Rat IL-4 ELISA Kit (AB46073)
  • sELISA

Supplier Data

Sandwich ELISA - Rat IL-4 ELISA Kit (AB46073)

Representative Standard Curve using ab46073

Key facts

検出方法

Colorimetric

サンプルタイプ

Cell culture supernatant, Serum

交差種

Rat

アッセイタイプ

Sandwich (quantitative)

検出感度

< 15 pg/mL

検出範囲

31.25 - 1000 pg/mL

アッセイ時間

4h 45m

アッセイプラットフォーム

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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製品の詳細

Abcam's IL-4 Rat in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-4 in cell culture supernatants, buffered solutions and plasma.

A monoclonal antibody specific for IL-4 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-4 concentRations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-4 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substRate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentRation of IL-4 present in the samples.

This kit will recognize both endogenous and recombinant rat IL-4.

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精度

[ { "reproducibilityType": "Inter", "sample": "Sample A", "replicates": 6, "mean": "128.6 pg/mL", "standardDeviation": "7.6", "coefficientOfVariability": "5.9" }, { "reproducibilityType": "Inter", "sample": "Sample B", "replicates": 6, "mean": "532.2 pg/mL", "standardDeviation": "20.6", "coefficientOfVariability": "3.8" }, { "reproducibilityType": "Intra", "sample": "Sample A", "replicates": 18, "mean": "379.3 pg/mL", "standardDeviation": "13.5", "coefficientOfVariability": "2.5" }, { "reproducibilityType": "Intra", "sample": "Sample B", "replicates": 19, "mean": "532.5 pg/mL", "standardDeviation": "5.99", "coefficientOfVariability": "1.6" } ]
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製品内容

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出荷温度及び保存条件

出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
保管に関する情報
+4°C
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製品プロトコール

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ターゲットの情報

Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4.
See full target information Il4
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websiteProtocolBooklet
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