Ras GTPase ELISA Kit (Chemiluminescent)

製品の詳細

Ras GTPase ELISA Kit (Chemiluminescent) ab134640 is a sandwich ELISA to measure Ras GTPase (Chemiluminescent) in cell culture extracts, tissue extracts with a sensitivity of 3000 ng/well.

How the assay works:

Ras GTPase ELISA Kits contain a Raf-RBD protein fused to GST that will be coated onto the provided 96-well (12 x 8 well plate), glutathione-coated plate. The activated Ras contained in cellular extract specifically binds to Raf-RBD, while inactive Ras does not bind. Bound Ras is detected by incubating with a primary antibody that detects H-Ras in mouse and H- & K-Ras in human samples. Addition of a secondary antibody conjugated to horseradish peroxidase (HRP) and developing solution provides a sensitive chemiluminescent readout that is easily quantified by luminescence. The 96-well plate with individual plate of 12 strips is suitable for manual use or high-throughput screening applications.

Assay Specificity

Ras GTPase ELISA Kit specifically detects activated H- and K-Ras in Human and H-Ras in rodent samples.

Ras GTPase ELISA Kit (Chemiluminescent) ab134640 protocol summary

1. Wash each well. Add sample to appropriate wells. Activated Ras binds Raf-RBD, inactive Ras does not. Incubate at room temperature.
2. Wash each well.
3. Add primary detection antibody. Incubate at room temperature.
4. Wash each well.
5. Add HRP-conjugated secondary antibody, which binds the primary antibody. Incubate at room temperature.
6. Wash each well. Add Chemiluminescent reagent.
7. Read using a luminometer.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.