Rabbit IgM ELISA Kit
Rabbit IgM ELISA Kit
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(2 Publications)
Rabbit IgM ELISA Kit is a sandwich ELISA designed to quantify Rabbit IgM with a sensitivity of 1.685 ng/mL.
- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 6.25 - 200 ng/mL
別名を表示する
Ig mu chain C region secreted form
- sELISA
Supplier Data
Sandwich ELISA - Rabbit IgM ELISA Kit (AB190539)
Representative standard curve using ab190539 IgM Rabbit ELISA Kit
組合せ製品
Reactivity data
製品の詳細
How the assay works
In this assay the IgM present in samples reacts with the anti-IgM antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-IgM antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound IgM. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgM in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgM in the test sample. The quantity of IgM in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details
Rabbit IgM ELISA Kit ab190539 protocol summary
1. Add standard or sample to each well used. Incubate at room temperature.
2. Aspirate and wash each well.
3. Add prepared HRP labeled secondary detector antibody. Incubate at room temperature
4. Aspirate and wash each well.
5. Add Chromogen Substrate Solution to each well.
6. Immediately begin recording the color development
精度
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製品内容
出荷温度及び保存条件
出荷温度
短期保存温度
長期保存温度
保管に関する情報
補足情報
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
IgM acts as the first line of defense during the initial stages of an immune response. It plays an important part in agglutination and neutralization of pathogens forming a complex that alerts other components of the immune system to take action. IgM exists before class-switching to IgG providing immediate protection. It also plays a role in antibody-dependent cellular cytotoxicity. Although not typically a long-term solution its presence is critical for providing initial protection until more specific antibodies like IgG and IgA are produced.
Pathways
The immune response that involves IgM begins with the activation of the classical complement pathway. This pathway bridges the innate and adaptive immunity ensuring the communication and coordination of immune responses. The pathway involves C1q protein binding to IgM-antigen complexes. IgM also interacts with antigen-presenting cells facilitating the processing of antigens and interaction with T cells. These interactions connect IgM with other proteins like IgG and IgA influencing various immune response pathways.
文献 (2)
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Animal science journal = Nihon chikusan Gakkaiho 92:e13560 PubMed34029409
2021
Applications
Unspecified application
Species
Unspecified reactive species
Journal of animal physiology and animal nutrition 104:196-203 PubMed31701584
2019
Applications
Unspecified application
Species
Unspecified reactive species
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